关键词: 哮喘, c-Jun氨基末端激酶, 气道炎症, 气道重塑

Abstract: Abastract：Objective To explore the dynamic changes and significance of phosphorylated c-Jun N－terminal kinase (P-JNK) in lung tissues of asthma rats. Methods Eighty male SD rats were randomly divided into the control group(group C) and asthma group(group A). According to the different phase time after the last challenge, the latter included animals with asthma for 0.5h、1h、2h、3h、6h、12h、24h. The histopathology and ultrastructure changes of pulmonary tissues were observed by light microscope and transmission electron microscope(TEM). The total brochial wall thickness (Wat) and the airway smooth muscle thickness(Wam) were measured by image analysis system. The protein expression of P-JNK in lung tissues was detected by immunohistochemistry(IHC) and Western blotting. Results HE staining of lung tissues from group A showed EOS infiltration, TEM showed basement membrane thickening, collagen proliferation. Wat and Wam in group A (to choose A1h) were significantly increased. The dynamic expression of P-JNK in group A lung tissues: the amounts of P-JNK were increased at the 0.5h(P＜0.01), temporarily decreased at the 2h, and significantly elevated up to peak at 3-6 h, then decreased at the 12h and retured to near baseline at the 24 h(P＞0.05). Conclusion The dynamic expressions of P-JNK in rats lung tissues of group A show that a course from fluctuatory elevation to recovery after OVA challenge, which implies that phosphrylation of JNK plays an important role in asthma airway inflammation and airway remodeling.

Key words: asthma, c-Jun N－terminal kinase, airway inflammation, airway remodeling