露蜂房蛋白抑制大鼠支气管平滑肌细胞增殖的可能机制

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (7): 683-687.

露蜂房蛋白抑制大鼠支气管平滑肌细胞增殖的可能机制

汪长东陈鹏闫旭何光源

华中科技大学

收稿日期:2008-06-02 修回日期:2008-09-07 出版日期:2009-07-20 发布日期:2009-07-20

Prossible mechanism of Nidus Vespae protein (1) inhibits proliferation of bronchial smooth muscle cell of rats

Chang-dong WANG, Peng CHEN, Xu YAN, Guang-yuan HE

Huazhong University of Science & Technology

Received:2008-06-02 Revised:2008-09-07 Online:2009-07-20 Published:2009-07-20

摘要/Abstract

摘要： 目的观察露蜂房蛋白[NVP(1)]对大鼠支气管平滑肌细胞增殖的抑制作用及其可能机制。方法用原代细胞培养，MTT法, 流式细胞术和免疫印迹法（Western- blotting）检测细胞增殖。结果 6.4×10-3g/L的NVP(1)明显抑制支气管平滑肌细胞增殖，并且阻断在细胞周期的G1期(对照组G1期为64.6％，NVP(1)处理组为90.5％) 。NVP(1)能增强细胞周期蛋白激酶抑制剂（p21）蛋白的表达，降低细胞周期蛋白激酶（cdk2）蛋白的表达。结论NVP(1)阻断支气管平滑肌细胞增殖于细胞周期的G1期。

关键词: NVP(1) 抑制支气管平滑肌细胞增殖机制

Abstract: Objective NVP (1) was one pure protein that isolated from Nidus Vespae. We found that NVP (1) can inhibit proliferation of bronchial smooth muscle cell. At the same time, we investigated the mechanism of NVP (1) in inhibiting proliferation of bronchial smooth muscle cell. Method Primary cell culture. The effects of NVP (1) on bronchial smooth muscle cells proliferation by MTT assay. The cell cycle was detected by flow cytometry. Western-blotting was used to reveal p21, cdk2 protein expression. Results NVP(1) (6.4×10-3g/L) can arrest bronchial smooth muscle cell in cycle G1 and increase the expression level of p21. On the contrary, NVP (1) can inhibit proliferation of bronchial smooth muscle cell by down-regulating cdk2 protein expression. Conclusion NVP (1) inhibits proliferation of bronchial smooth muscle cells and induces G1 cell cycle arrest.

Key words: The mechanism of Nidus Vespae protein 1（NVP 1）inhibits proliferation of bronchial smooth muscle cell

汪长东陈鹏闫旭何光源. 露蜂房蛋白抑制大鼠支气管平滑肌细胞增殖的可能机制[J]. 基础医学与临床, 2009, 29(7): 683-687.

Chang-dong WANG; Peng CHEN; Xu YAN; Guang-yuan HE. Prossible mechanism of Nidus Vespae protein (1) inhibits proliferation of bronchial smooth muscle cell of rats[J]. Basic & Clinical Medicine, 2009, 29(7): 683-687.