PP60C-SRC/ERK1/2信号通路调节大鼠精原干细胞活性

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (7): 678-682.

PP60C-SRC/ERK1/2信号通路调节大鼠精原干细胞活性

陈加祥徐斯凡王晶磊杨俊玲秦海燕邹挺

南昌大学医学院中央民族大学生命与环境科学学院南昌大学医学院

收稿日期:2008-06-06 修回日期:2008-09-03 出版日期:2009-07-20 发布日期:2009-07-20
通讯作者: 王晶磊

PP60C-SRC/ERK1/2 regulates the viability of rat spermatogonial stem cells in vitro

Jia-xiang CHEN, Si-fan XU, Jing-lei WANG, Jun-ling YANG, Hai-yan QIN, Ting ZOU

Medical College of Nanchang University Life and Environmental Science College, Central University for National Minorities Medical College of Nanchang University

Received:2008-06-06 Revised:2008-09-03 Online:2009-07-20 Published:2009-07-20
Contact: Jing-lei WANG,

摘要/Abstract

摘要： 目的研究PP60C-SRC通过磷酸化的细胞外调节激酶1/2 (p-ERK1/2)调节体外培养的9日龄大鼠精原干细胞的活性。方法用MTT观察反义C-SRC脱氧寡核苷酸(ODNs)及PD98059对精原干细胞活性的影响；用Western blot检测PP60C-SRC和p-ERK1/2的表达。结果与空白对照组相比，10 ?mol/L反义C-SRC ODNs作用12 h后精原干细胞活性下降了8.1 % (P＜0.05)，10 ?mol/L PD98059作用24 h后精原干细胞活性下降了9.4 %(P＜0.01)。反义C-SRC ODNs组PP60C-SRC、p-ERK1/2蛋白表达与空白对照组相比分别下降了33.8 %和45.3 % (均P<0.01)；10 ?mol/L PD98059作用精原干细胞24 h后，p-ERK1/2蛋白表达下降了34.5 %。结论 PP60C-SRC可能通过p-ERK1/2蛋白而调节精原干细胞的活性。

关键词: 大鼠, 精原干细胞, pp60c-src, 细胞外调节激酶1/2

Abstract: Objective The present study aimed to investigate the effect of PP60C-SRC on the viability of rat spermatogonial stem cells in 9 day-old rat. Methods MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense C-SRC oligodeoxynucleotides (ODNs) and PD98059 in vitro; Western blot was used to observe the PP60C-SRC and phosphorylated extracellular regulated kinase1/2 (p-ERK1/2) contents. Results Compared with that in control group, the viability of the cells decreased by 8.1 % (P<0.05) and 9.4 %(P＜0.01), respectively, after the spermatogonial stem cells were treated with antisense C-SRC ODNs and PD98059. Compared with the control group, the PP60C-SRC and p-ERK1/2 contents decreased by 33.8 % and 38.4 % (both P<0.01), respectively, in the spermatogonial stem cells after transfected antisense C-SRC ODNs. Compared with the control group, the p-ERK1/2 contents decreased by 34.5 % (P<0.01) in the spermatogonial stem cells after treated with PD98059. Conclusion PP60C-SRC regulates the viability of rat spermatogonia stem cells through p-ERK1/2.

Key words: rat, spermatogonial stem cells, pp60c-src, ERK1/2

中图分类号:

Q492

陈加祥徐斯凡王晶磊杨俊玲秦海燕邹挺. PP60C-SRC/ERK1/2信号通路调节大鼠精原干细胞活性[J]. 基础医学与临床, 2009, 29(7): 678-682.

Jia-xiang CHEN; Si-fan XU; Jing-lei WANG; Jun-ling YANG; Hai-yan QIN; Ting ZOU. PP60C-SRC/ERK1/2 regulates the viability of rat spermatogonial stem cells in vitro[J]. Basic & Clinical Medicine, 2009, 29(7): 678-682.

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