上调miR-200c-5p的表达抑制人结直肠癌细胞系SW480增殖

doi:10.16352/j.issn.1001-6325.2023.02.241

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (2): 241-245.doi: 10.16352/j.issn.1001-6325.2023.02.241

上调miR-200c-5p的表达抑制人结直肠癌细胞系SW480增殖

王芳芳, 曹慧媛, 汪婧, 王宁, 黄国良^*

广东医科大学中美肿瘤所东莞市表观遗传学重点实验室广东医科大学附属东莞第一医院广东省医学分子诊断重点实验室,广东东莞 523808

收稿日期:2022-05-20 修回日期:2022-07-21 出版日期:2023-02-05 发布日期:2023-02-02
通讯作者: ^*huangguoliang@gdmu.edu.cn
基金资助:
国家自然科学基金(81772982); 广东省高校科研平台和科研项目基金(2019KTSCX049)

Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480

WANG Fangfang, CAO Huiyuan, WANG Jing, WANG Ning, HUANG Guoliang^*

Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, the First Dongguan Affiliated Hospital, Key Laboratory for Epigenetics of Dongguan City, China-America Cancer Research Institute, Guangdong Medical University, Dongguan 523808, China

Received:2022-05-20 Revised:2022-07-21 Online:2023-02-05 Published:2023-02-02
Contact: ^*huangguoliang@gdmu.edu.cn

摘要/Abstract

摘要： 目的探讨miR-200c-5p对人结直肠癌细胞系SW480恶性增殖的影响。方法将miR-200c-5p模拟物/抑制剂及阴性对照分别瞬时转染人结直肠癌细胞系SW480。通过qPCR检测miR-200c-5p的表达,利用CCK-8法和平板克隆形成试验检测miR-200c-5p对结直肠癌细胞增殖活力和克隆形成能力的影响。使用生物信息学方法分析miR-200c-5p在结直肠癌中的差异靶基因和相关通路。结果瞬时转染miR-200c-5p模拟物/抑制剂成功高/低表达miR-200c-5p(P＜0.05);高表达miR-200c-5p细胞增殖活力降低,低表达则相反(P＜0.05);高表达miR-200c-5p的细胞克隆形成率低于对照组细胞,低表达则相反(P＜0.05);miR-200c-5p的靶基因CXCL10在结直肠癌细胞中显著上调,C2orf72、ZC3H12C和DST显著下调,miR-200c-5p显著相关的KEGG通路为“melanoma”、 “microRNAs in cancer”和“bladder cancer”。结论 miR-200c-5p抑制人结直肠癌细胞系SW480的增殖及克隆形成,与结直肠癌的发生、发展相关。

关键词: 结直肠癌, miR-200c-5p, 细胞增殖

Abstract: Objective To investigate the effect of miR-200c-5p on malignant proliferation of human colorectal cancer cell line SW480. Methods Human colorectal cancer cell line SW480 was transfected with miR-200c-5p mimic/inhibitor and negative control agent respectively. The expression of miR-200c-5p was detected by qPCR, and the effects of miR-200c-5p on the proliferation activity and clonogenesis ability of colorectal cancer cells were detected by CCK-8 and foci formation experiment. Bioinformatics methods were used to analyze the differential target genes and related pathways of miR-200c-5p in human colorectal cancer. Results Transient transfection with miR-200c-5p mimic/inhibitor succeeded in high/low expression of miR-200c-5p (P＜0.05). The proliferation of cells with high expression of miR-200c-5p was significantly inhibited as compare to that of controls(P＜0.05). The clone-formation rate of miR-200c-5p cells with high expression was significantly lower than that of untransfected cells, while the clone-formation rate of miR-200c-5p cells with low expression was the opposite (P＜0.05). CXCL10, the target gene of miR-200c-5p, was significantly up-regulated in colorectal cancer, while C2orf72, ZC3H12C and DST were significantly down-regulated. The most significant KEGG pathways were “melanoma”, “microRNAs in cancer” and “bladder cancer”. Conclusions miR-200c-5p inhibits the proliferation and clonal formation of human colorectal cancer cell line SW480, which is related to the occurrence and progression of colorectal cancer.

Key words: colorectal cancer, miR-200c-5p, cell proliferation

中图分类号:

R363.1

王芳芳, 曹慧媛, 汪婧, 王宁, 黄国良. 上调miR-200c-5p的表达抑制人结直肠癌细胞系SW480增殖[J]. 基础医学与临床, 2023, 43(2): 241-245.

WANG Fangfang, CAO Huiyuan, WANG Jing, WANG Ning, HUANG Guoliang. Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480[J]. Basic & Clinical Medicine, 2023, 43(2): 241-245.

参考文献 12

[1]	Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68: 394-424.
[2]	Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66: 115-132.
[3]	Lu TX, Rothenberg ME. MicroRNA[J]. J Allergy Clin Immunol, 2018, 141: 1202-1207.
[4]	Wang Z. The guideline of the design and validation of miRNA mimics[J]. Methods Mol Biol, 2011, 676: 211-223.
[5]	Huang GL, Sun J, Lu Y, et al. miR-200 family and cancer: from a meta-analysis view[J]. Mol Aspects Med, 2019, 70: 57-71.
[6]	武坤, 聂波, 李云涛, 等. 外周血miR-200c水平对移植异基因造血干细胞后急性移植物抗宿主病发生的预测作用[J]. 基础医学与临床, 2022, 42: 871-875.
[7]	Sengupta D, Deb M, Kar S, et al. Dissecting miRNA facilitated physiology and function in human breast cancer for therapeutic intervention[J]. Semin Cancer Biol, 2021, 72: 46-64.
[8]	Paterson EL, Kazenwadel J, Bert AG, et al. Down-regulation of the miRNA-200 family at the invasive front of colorectal cancers with degraded basement membrane indicates EMT is involved in cancer progression[J]. Neoplasia, 2013, 15: 180-191.
[9]	Karin N, Razon H. Chemokines beyond chemo-attraction: CXCL10 and its significant role in cancer and autoim-munity[J]. Cytokine, 2018, 109: 24-28.
[10]	Khorasani M, Shahbazi S, Hosseinkhan N, et al. Analysis of differential expression of microRNAs and their target genes in prostate cancer: a bioinformatics study on microarray gene expression data[J]. Int J Mol Cell Med, 2019, 8: 103-114.
[11]	Liu L, Zhou Z, Huang S, et al. Zc3h12c inhibits vascular inflammation by repressing NF-κB activation and pro-inflammatory gene expression in endothelial cells[J]. Biochem J, 2013, 451: 55-60.
[12]	Leick KM, Rodriguez AB, Melssen MM, et al. The barrier molecules junction plakoglobin, filaggrin, and dystonin play roles in melanoma growth and angiogenesis[J]. Ann Surg, 2019, 270: 712-722.

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上调miR-200c-5p的表达抑制人结直肠癌细胞系SW480增殖

Up-regulation of miR-200c-5p inhibits proliferation of human colorectal cancer cell line SW480

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