基础医学与临床 ›› 2022, Vol. 42 ›› Issue (10): 1561-1566.doi: 10.16352/j.issn.1001-6325.2022.10.1561

• 研究论文 • 上一篇    下一篇

EphB2受体在脑缺血模型大鼠功能恢复不同阶段中的表达

张洁婷, 李海僖, 王婷婷, 云璐, 杨海*   

  1. 青岛市中心医院 药学部, 山东 青岛 266042
  • 收稿日期:2021-09-14 修回日期:2022-02-18 出版日期:2022-10-05 发布日期:2022-09-23
  • 通讯作者: * yanghqd79@163.com
  • 基金资助:
    青岛市医药科研指导计划(2019-WJZD063);青岛中医科研计划(2019-zyy033)

Expression of EphB2 receptor at different stages of functional recovery in cerebral ischemia rats

ZHANG Jie-ting, LI Hai-xi, WANG Ting-ting, YUN Lu, YANG Hai*   

  1. Department of Pharmacy, Qingdao Central Hospital, Qingdao 266042, China
  • Received:2021-09-14 Revised:2022-02-18 Online:2022-10-05 Published:2022-09-23
  • Contact: * yanghqd79@163.com

摘要: 目的 初步探讨促红细胞生成素产生肝细胞B2(EphB2)受体在大鼠大脑中动脉栓塞(MCAO)后功能恢复不同阶段中的作用。方法 将64只大鼠分为假手术组、MCAO造模后2、14 和28 d共4组,改良型mNSS评分评价大鼠神经运动功能恢复情况,q-PCR检测EphB2 mRNA表达,Western blot检测EphB2受体蛋白表达。结果 与假手术组相比,MCAO术后2 d时mNSS评分最高(P<0.001),后随时间增加而逐渐降低;MCAO术后2 d时海马区EphB2受体表达明显增加(P< 0.05),后随时间增加而逐渐降低,且其与mNSS总评分具有相关性。各组皮质区EphB2受体及海马区EphB2 mRNA在脑缺血后功能恢复过程中均未见显著改变。结论 MCAO造模后大鼠海马区EphB2受体表达先上调后逐渐降低,与功能恢复情况密切相关。

关键词: EphB2受体, 脑缺血, 功能恢复, 不同阶段

Abstract: Objective To investigate the expression of erythropoietin-producing hepatocyte B2 (EphB2) receptor at different phases of functional recovery after middle cerebral artery occlusion(MCAO) in rats. Methods Sixty-four rats were divided into four groups:sham group and 2, 14 and 28 days after MCAO. The neurological and motor function was evaluated with a modified mNSS score at 2 d, 14 d or 28 d after surgery. The mRNA and protein in the hippocampus and cortex were measured by q-PCR and Western blot, respectively. Results Rats showed the highest mNSS score at 2 d after MCAO as compared with sham group (P<0.001) and the score decreased with time.The protein level of EphB2 receptor in the hippocampus increased significantly at 2 d after MCAO (P<0.05)compared with sham group, and then gradually decreased at 14 and 28 d after MCAO. The expression of EphB2 receptor showed correlation with mNSS scores. However, the expression of EphB2 receptors in the cortex and the EphB2 mRNA level in the hippocampus did not change significantly after MCAO. Conclusions The expression of EphB2 receptor in the hippocampus is up-regulated at early phase of MCAO and then decreased, which is closely correlated with functional score in rats.

Key words: EphB2 receptor, cerebral ischemia, functional recovery, different stages

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