Fc-gamma Ⅰ型受体在阿尔茨海默病患者和模型小鼠脑皮质的表达

doi:10.16352/j.issn.1001-6325.2022.05.004

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (5): 740-744.doi: 10.16352/j.issn.1001-6325.2022.05.004

Fc-gamma Ⅰ型受体在阿尔茨海默病患者和模型小鼠脑皮质的表达

孙健茹, 仇文颖, 钱晓菁, 马超^*

中国医学科学院基础医学研究所北京协和医学院基础学院人体解剖与组织胚胎学系, 北京 100005

收稿日期:2022-01-13 修回日期:2022-03-23 出版日期:2022-05-05 发布日期:2022-04-28
通讯作者: * machao@ibms.cams.cn
基金资助:
国家自然科学基金(81771205);原创探索计划项目(82050004);中国医学科学院医学与健康科技创新工程项目(CIFMS #2021-1-I2M-025)

Expression of Fc-gamma receptor type Ⅰ in brain cortex of patients with Alzheimer's disease and the mouse model

SUN Jian-ru, QIU Wen-ying, QIAN Xiao-jing, MA Chao^*

Department of Anatomy,Histology and Embryology,Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC,Beijing 100005,China

Received:2022-01-13 Revised:2022-03-23 Online:2022-05-05 Published:2022-04-28
Contact: * machao@ibms.cams.cn

摘要/Abstract

摘要： 目的探讨高亲和力IgG受体Fc-gamma Ⅰ型(FcγRⅠ)在阿尔茨海默病(AD)脑皮层的表达变化。方法选用正常人脑组织样本和神经病理诊断为AD患者脑组织样本,6月龄野生型小鼠和APP/PS1小鼠。免疫荧光和免疫蛋白印迹检测脑皮层FcγRⅠ的细胞定位和表达。结果人和小鼠脑皮层的神经元均普遍表达FcγRⅠ。与健康人脑相比,AD患者脑皮层的FcγRⅠ表达显著降低(P＜0.05)。与野生型小鼠脑相比,AD模型小鼠脑皮层的FcγRⅠ表达也呈现出显著降低(P＜0.05)。结论 IgG高亲和力受体FcγRⅠ在AD患者脑和AD模型小鼠脑皮层的表达显著下降,可能与AD的神经病理机制相关。

关键词: 阿尔茨海默病(AD), 神经元, 免疫球蛋白G(IgG), Fc-gammaⅠ型受体(FcγRⅠ)

Abstract: Objective To explore expression changes of high affinity IgG receptor Fc-gamma Ⅰ type(FcγRⅠ) in brain cortex of patients with Alzheimer's disease(AD). Methods Normal human brain tissue samples and brain tissue samples with neuropathological diagnosis of AD, wild type mice and APP/PS1 mice were used. Immunofluorescence and Western blot were used to define cellular localization and expression of FcγRⅠ in cerebral cortex. Results FcγRⅠ was generally expressed in neurons of human and mouse cerebral cortex. Compared with normal human brain, FcγRⅠ expression in AD human brain cortex decreased significantly(P＜0.05). Compared with wild type mouse brain, FcγRⅠ expression in cerebral cortex of AD mouse models also decreased significantly(P＜0.05). Conclusions The expression of IgG high affinity receptor FcγRⅠ in AD human brain and cerebral cortex of AD mouse models decreases significantly that suggestes that FcγRⅠ is potentially correlated with AD neuropathological pathogenesis.

Key words: Alzheimer's disease (AD), neuron, immunoglobulin G(IgG), Fc-gamma receptor type Ⅰ (FcγRⅠ)

中图分类号:

R322.81

孙健茹, 仇文颖, 钱晓菁, 马超. Fc-gamma Ⅰ型受体在阿尔茨海默病患者和模型小鼠脑皮质的表达[J]. 基础医学与临床, 2022, 42(5): 740-744.

SUN Jian-ru, QIU Wen-ying, QIAN Xiao-jing, MA Chao. Expression of Fc-gamma receptor type Ⅰ in brain cortex of patients with Alzheimer's disease and the mouse model[J]. Basic & Clinical Medicine, 2022, 42(5): 740-744.

参考文献

[1] Scheltens P, Strooper BD, Kivipelto M, et al. Alzheimer's disease[J]. The Lancet, 2021, 397: 1577-1590.
[2] Bournazos S. IgG Fc receptors: evolutionary considera-tions[J]. Curr Top Microbiol Immunol, 2019, 423: 1-11.
[3] Stamou M, Grodzki AC, Oostrum MV, et al. Fc gamma receptors are expressed in the developing rat brain and activate downstream signaling molecules upon cross-linking with immune complex[J]. J Neuroinflammation, 2018, 15: 7-29.
[4] 刘帆, 苏思, 王涛, 等. 敲除背根神经节Fcgr1减弱类风湿关节炎模型大鼠NF-κB/NLRP3通路活化[J]. 基础医学与临床, 2021, 41: 963-969.
[5] 刘帆, 李春艳, 徐鲁斌, 等. Fcγ和Fcε受体在小鼠三叉神经节的表达[J]. 基础医学与临床, 2015, 35: 729-733.
[6] Nimmerjahn F, Ravetch JV. Fc gamma receptors: old friends and new family members[J]. Immunity, 2006, 24: 19-28.
[7] Fernandez-Vizarra P, Lopez-Franco O, Mallavia B, et al. Immunoglobulin G Fc receptor deficiency prevents Alzheimer-like pathology and cognitive impairment in mice[J]. Brain, 2012, 135: 2826-2837.
[8] Kam TI, Song S, Gwon Y, et al. Fc gamma RIIb mediates amyloid-beta neurotoxicity and memory impair-ment in Alzheimer's disease[J]. J Clin Invest, 2013, 123: 2791-2802.
[9] Kam TI, Park H, Gwon Y, et al. Fc gamma RIIb-SHIP2 axis links A beta to tau pathology by disrupting phosphoinositide metabolism in Alzheimer's disease model[J]. Elife, 2016, 5: e18691.doi:10.7554/eLife.18691.
[10] Ravetch JV, Bolland S. IgG Fc receptors[J]. Annu Rev Immunol, 2001, 19: 275-290.
[11] Peress NS, Fleit HB, Perillo E, et al. Identification of FcγRⅠ, Ⅱ and Ⅲ on normal human brain ramified microglia and on microglia in senile plaques in Alzheimer's disease[J]. J Neuroimmunol, 1993, 48: 71-79.
[12] Wilcock DM, Rojiani A, Rosenthal A, et al. Passive amyloid immunotherapy clears amyloid and transiently activates microglia in a transgenic mouse model of amyloid deposition[J]. J Neurosci, 2004, 24: 6144-6151.
[13] Zotova E, Bharambe V, Cheaveau M, et al. Inflammatory components in human Alzheimer's disease and after active amyloid-beta42 immunization[J]. Brain, 2013, 136: 2677-2696.
[14] Bard F, Cannon C, Barbour R, et al. Peripherally administered antibodies against amyloid beta-peptide enter the central nervous system and reduce pathology in a mouse model of Alzheimer disease[J]. Nat Med, 2000, 6: 916-919.

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Fc-gamma Ⅰ型受体在阿尔茨海默病患者和模型小鼠脑皮质的表达

Expression of Fc-gamma receptor type Ⅰ in brain cortex of patients with Alzheimer's disease and the mouse model

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