胃癌患者血清IL-1的升高和癌组织JAK2/STAT3信号通路的激活

doi:10.16352/j.issn.1001-6325.2022.03.035

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (3): 401-405.doi: 10.16352/j.issn.1001-6325.2022.03.035

胃癌患者血清IL-1的升高和癌组织JAK2/STAT3信号通路的激活

牛世伟^1,2, 苏韫^1,2*, 龚红霞^1,2, 李婷婷^1,2, 曾元丁^1,2, 张晗^1,2, 聂蓬³

甘肃中医药大学 1.基础医学院;
2.甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000;
3.甘肃省武威肿瘤医院武威医学科学院胃外科, 甘肃武威 733000

收稿日期:2021-03-04 修回日期:2021-08-13 出版日期:2022-03-05 发布日期:2022-03-04
通讯作者: ^* suyungszy@126.com
基金资助:
国家自然科学基金(81660744);甘肃省中医方药挖掘与创新转化重点实验室基金(ZYFY-2020-004);甘肃中医药大学中西医结合基础学科科研培育项目[(5)2020—2021年度];敦煌医学与转化教育部重点实验室基金(DHYX19-08)

Increased serum IL-1 level and activated JAK2/STAT3 signaling pathway of cancer tissue in patients with gastric cancer

NIU Shi-wei^1,2, SU Yun^1,2*, GONG Hong-xia^1,2, LI Ting-ting^1,2, ZENG Yuan-ding^1,2, ZHANG Han^1,2, NIE Peng³

1. School of Basic Medicine;
2. Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and Study on Prevention, Gansu University of Traditional Chinese Medicine, Lanzhou 730000;
3. Department of Gastric Surgery, Wuwei Academy of Medical Sciences, Gansu Province Wuwei Cancer Hospital, Wuwei 733000, China

Received:2021-03-04 Revised:2021-08-13 Online:2022-03-05 Published:2022-03-04
Contact: ^* suyungszy@126.com

摘要/Abstract

摘要： 目的探讨胃癌患者血清中白细胞介素1(IL-1)含量升高及癌组织中JAK2/STAT3信号通路激活在胃癌生长过程中的作用。方法选取甘肃省武威肿瘤医院胃癌手术患者及同期健康体检者各30例;酶联免疫吸附法(ELISA)检测样本血清中IL-1和IL-10的含量;免疫组化和Western blot分别检测各组织中Bcl-2、Bax及JAK2、STAT3、pJAK2、pSTAT3、Bcl-2和Bax的含量。实时荧光定量PCR检测各组织中JAK2、STAT3、Bcl-2和Bax基因的含量。结果与对照组相比,胃癌患者血清中IL-1含量上升,而IL-10含量下降(P＜0.05);Bcl-2在癌组织中的含量明显高于对照组和癌旁组,而Bax含量相反(P＜0.05)。与对照组相比,癌组织和癌旁组织中JAK2、STAT3、pJAK2、pSTAT3和Bcl-2蛋白含量增加,而Bax含量减少(P＜0.05)。与癌旁组织相比,癌组织中JAK2、STAT3、pJAK2、pSTAT3和Bcl-2蛋白含量增加,而Bax含量减少(P＜0.05)。与对照组相比,癌组织和癌旁组织中JAK2、STAT3和Bcl-2 mRNA含量增加,而Bax mRNA含量减少(P＜0.05)。与癌旁组织相比,癌组织中JAK2、STAT3和Bcl-2 mRNA含量增加,而Bax mRNA含量减少(P＜0.05)。结论促炎因子IL-1的异常升高及JAK2/STAT3信号通路的异常激活与胃癌的发生发展关系密切。

关键词: 胃癌, JAK2/STAT3信号通路, IL-1, IL-10

Abstract: Objective To explore the role of elevated serum interleukin 1(IL-1) levels in patients with gastric cancer and activation of JAK2/STAT3 signaling pathway in tissues of gastric cancer. Methods A total of 30 gastric cancer surgery patients and 30 healthy subjects were selected from Wuwei Cancer Hospital of Gansu Province; The levels of IL-1 and IL-10 in serum was determined by enzyme-linked immunosorbent assay (ELISA); Immunohistochemistry and Western blot were used to detect the contents of Bcl-2, Bax and JAK2,STAT3, pJAK2, pSTAT3, Bcl-2, Bax in tissues. The contents of JAK2, STAT3, Bcl-2 and Bax genes were measured by RT-qPCR. Results Compared with the control group, the level of IL-1 in the serum of patients with gastric cancer was up-regulated, and the level of IL-10 was down-regulated(P＜0.05); The content of Bcl-2 in cancer tissue was significantly higher than that in the normal group and the adjacent group, but the Bax content showed an opposite profile(P＜0.05). Compared with the control group, JAK2, STAT3, pJAK2, pSTAT3 and Bcl-2 protein content in cancer tissue and adjacent tissues were increased, while the content of Bax was decreased(P＜0.05). Compared with the adjacent tissues, the JAK2, STAT3, pJAK2, pSTAT3 and Bcl-2 protein contents in cancer tissues increased, while the Bax content was decreased(P＜0.05). Compared with the control group, the contents of JAK2, STAT3 and Bcl-2 mRNA in cancer tissues and adjacent tissues were increased, while Bax mRNA was decreased(P＜0.05). Compared with adjacent tissues, the contents of JAK2, STAT3 and Bcl-2 mRNA in cancer tissues increased, while the content of Bax mRNA was decreased(P＜0.05). Conclusions Abnormal elevation of proinflammatory factors and abnormal activation of JAK2/STAT3 signaling pathway are closely related to the occurrence and development of gastric cancer.

Key words: gastric cancer, JAK2/STAT3 signaling pathway, IL-1, IL-10

中图分类号:

R735.2

牛世伟, 苏韫, 龚红霞, 李婷婷, 曾元丁, 张晗, 聂蓬. 胃癌患者血清IL-1的升高和癌组织JAK2/STAT3信号通路的激活[J]. 基础医学与临床, 2022, 42(3): 401-405.

NIU Shi-wei, SU Yun, GONG Hong-xia, LI Ting-ting, ZENG Yuan-ding, ZHANG Han, NIE Peng. Increased serum IL-1 level and activated JAK2/STAT3 signaling pathway of cancer tissue in patients with gastric cancer[J]. Basic & Clinical Medicine, 2022, 42(3): 401-405.

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胃癌患者血清IL-1的升高和癌组织JAK2/STAT3信号通路的激活

Increased serum IL-1 level and activated JAK2/STAT3 signaling pathway of cancer tissue in patients with gastric cancer

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