基础医学与临床 ›› 2022, Vol. 42 ›› Issue (2): 260-267.doi: 10.16352/j.issn.1001-6325.2022.02.012

• 研究论文 • 上一篇    下一篇

下调lncRNA DNM3OS通过靶向miR-193a-3p增强人直肠癌细胞系SW-480的放疗敏感性

刘明胜*, 陈文超, 蔡颖畅   

  1. 衢州市人民医院 肛肠外科, 浙江 衢州 324000
  • 收稿日期:2020-10-28 修回日期:2021-06-08 出版日期:2022-02-05 发布日期:2022-01-24
  • 通讯作者: * msv946@163.com
  • 基金资助:
    衢州市指导性科技计划项目(20172005)

Down-regulation of lncRNA DNM3OS enhances radiotherapy sensitivity of human rectal cancer cell line SW-480 by targeting miR-193a-3p

LIU Ming-sheng*, CHEN Wen-chao, CAI Ying-chang   

  1. Department of Anorectal Surgery, Quzhou People's Hospital, Quzhou 324000, China
  • Received:2020-10-28 Revised:2021-06-08 Online:2022-02-05 Published:2022-01-24
  • Contact: * msv946@163.com

摘要: 目的 探讨lncRNA DNM3OS对直肠癌SW-480细胞的增殖、凋亡以及放射敏感性的影响及分子机制。方法 选取30例直肠癌患者癌组织及癌旁组织,RT-qPCR检测DNM3OS和miR-193a-3p的表达水平;将抑制DNM3OS的表达载体、过表达miR-193a-3p载体转染至SW-480细胞,将DNM3OS与抑制miR-193a-3p的表达载体共转染至SW-480细胞,并用4 Gy射线照射;细胞集落形成实验检测细胞放射敏感性;四甲基偶氮唑盐比色法(MTT)检测SW-480细胞增殖抑制率;流式细胞测量术检测SW-480细胞凋亡;双荧光素酶报告基因实验检测DNM3OS和miR-193a-3p的靶向调控。结果 直肠癌组织中DNM3OS表达水平高于癌旁组织,miR-193a-3p表达水平低于癌旁组织(P<0.05)。抑制DNM3OS表达或过表达miR-193a-3p后,SW-480细胞增殖抑制率和凋亡率升高(P<0.05);经射线照射后细胞存活分数降低,细胞增殖抑制率和凋亡率升高(P<0.05)。DNM3OS靶向调控miR-193a-3p,干扰miR-193a-3p表达逆转了抑制lncRNA DNM3OS表达对直肠癌SW-480细胞增殖、凋亡及放射敏感性的影响。结论 抑制lncRNA DNM3OS表达可通过靶向上调miR-193a-3p抑制SW-480细胞增殖,促进细胞凋亡以及增强细胞的放射敏感性。

关键词: lncRNA DNM3OS, miR-193a-3p, 直肠癌, 放射敏感性

Abstract: Objective To explore the effect of lncRNA DNM3OS on the proliferation, apoptosis and radio-sensitivity of rectal cancer SW-480 cells and its molecular mechanism. Methods The samples from cancerous tissues and adjacent tissues of 30 rectal cancer patients were selected and the expression of DNM3OS and miR-193a-3p was detected by RT-qPCR; Transfected the inhibited DNM3OS expression vector or over-expression miR-193a-3p vector into SW-480 cells and co-transfect DNM3OS and inhibited miR-193a-3p expression vector into SW-480 cells and then irradiated them with 4 Gy rays; Cell colony formation experiment was applined to detect cell radio-sensitivity; methyl thiazolyl tetrazolium assay (MTT) was used to detect cell proliferation inhibition rate; flow cytometry was used to detect cell apoptosis; The targeted regulation of DNM3OS and miR-193a-3p were examined by dual luciferase reporter gene assay. Results The expression of DNM3OS in rectal cancer tissue was higher than that in adjacent tissues and the expression of miR-193a-3p was lower than that in adjacent tissues (P<0.05). After inhibiting DNM3OS expression or over-expression of miR-193a-3p, SW-480 cell proliferation inhibition rate and apoptosis rate was increased(P<0.05); after radiation, cell survival score was decreased and cell proliferation inhibition rate as well as apoptosis rate were increased (P<0.05). DNM3OS targeted and regulated miR-193a-3p, and interference with miR-193a-3p expression reversed the effect of inhibiting lncRNA DNM3OS expression on the proliferation, apoptosis and radiosensitivity of rectal cancer SW-480 cells. Conclusions Down-regulation of expression of lncRNA DNM3OS can inhibit the proliferation of SW-480 cells by up-regulation of miR-193a-3p, thus to promote cell apoptosis and enhance cell radio-sensitivity.

Key words: lncRNA DNM3OS, miR-193a-3p, rectal cancer, radio sensitivity

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