多肽P7对肿瘤组织的靶向识别与Hsp90表达水平相关

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (7): 982-987.

多肽P7对肿瘤组织的靶向识别与Hsp90表达水平相关

孔德欣, 黄薇, 杨楠, 刘雁勇^*

中国医学科学院基础医学研究所北京协和医学院基础学院药理学系,北京 100005

收稿日期:2021-04-14 修回日期:2021-05-18 出版日期:2021-07-05 发布日期:2021-06-17
通讯作者: *yanyongliu@ibms.pumc.edu.cn
基金资助:
国家科技重大专项(2019ZX09301-170); 国家自然科学基金(81972688);北京市自然科学基金(7192128,7172134);中国医学科学院医学与健康科技创新工程(2016-I2M-3-004);协和青年科研基金与中央高校基本科研业务费专项基金(3332015113,2017350002)

Recognition of tumor-targeting peptide P7 related to the expression level of Hsp90

KONG De-xin, HUANG Wei, YANG Nan, LIU Yan-yong^*

Department of Pharmacology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, China

Received:2021-04-14 Revised:2021-05-18 Online:2021-07-05 Published:2021-06-17
Contact: *yanyongliu@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的分析热休克蛋白90(Hsp90)双功能靶向抑制多肽LPLTPLP(P7)对不同肿瘤组织及癌旁组织的识别效果,以及与Hsp90表达的相关性。方法通过免疫组化和免疫荧光染色法分别检测组织芯片中48例16种器官肿瘤及匹配或不匹配的边缘或癌旁组织的Hsp90表达水平以及FITC-P7的识别效果,并对两组数据进行相关性分析。结果在肿瘤组织中,Hsp90高表达且对FITC-P7的识别效果更好,两组数据呈现中度相关性(r=0.5147,P＜0.01)。结论 P7对肿瘤组织的识别与其Hsp90表达水平相关,证实了P7靶向Hsp90的能力。

关键词: 热休克蛋白90(Hsp90), 多肽, 相关性分析

Abstract: Objective To analyze the correlation between the expression of heat shock protein 90 (Hsp90) and the recognition of LPLTPLP (P7) on different tumor tissues and para-carcinoma tissues. Methods The expression of HSP90 and recognition effects of FITC-P7 in 48 cases of patients with 16 types of organ tumors and matched or mismatched marginal or adjacent tissues were analyzed by immunohistochemistry and immunofluorescence, respectively. Results Hsp90 was highly expressed in tumor tissues than para-carcinoma tissues. FITC-P7 showed a preferred recognition effect on tumor tissues in proportion to Hsp90 expression levels as determined by immunohistochemistry. There was moderate correlation in the two groups of data (r=0.5147,P＜0.01). Conclusions The recognition of tumor tissues by P7 is positively related to the expression level of Hsp90, suggesting the targeting of P7 at Hsp90.

Key words: heat shock protein 90(Hsp90), polypeptide, correlation analysis

中图分类号:

孔德欣, 黄薇, 杨楠, 刘雁勇. 多肽P7对肿瘤组织的靶向识别与Hsp90表达水平相关[J]. 基础医学与临床, 2021, 41(7): 982-987.

KONG De-xin, HUANG Wei, YANG Nan, LIU Yan-yong. Recognition of tumor-targeting peptide P7 related to the expression level of Hsp90[J]. Basic & Clinical Medicine, 2021, 41(7): 982-987.

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