青稞酥油饮食对小鼠肠道菌群以及葡聚糖硫酸钠诱导的小鼠结肠炎症状的影响

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (2): 203-208.

青稞酥油饮食对小鼠肠道菌群以及葡聚糖硫酸钠诱导的小鼠结肠炎症状的影响

李春美¹, 赵微¹, 解相宏¹, 张伟虹², 杨佳卉², 李军^3*, 刘晓军^1*

1.中国医学科学院北京协和医学院基础医学研究所医学分子生物学国家重点实验室,北京 100005;
2.山西医科大学基础医学院微生物学与免疫学教研室, 山西太原 030001;
3.北京大学第三医院消化科, 北京 100191

收稿日期:2020-09-14 修回日期:2020-10-28 出版日期:2021-02-05 发布日期:2021-01-19
通讯作者: ^*yanwt2003@163.com; xiaojunliu@ibms.pumc.edu.cn
基金资助:
中国医学科学院医学与健康科技创新工程(CIFMS2017-I2M-1-008)

Effects of barley plus butter dietary on symptoms of intestinal flora in mice and dextran sodium sulfate induced ulcerative colitis

LI Chun-mei¹, ZHAO Wei¹, XIE Xiang-hong¹, ZHANG Wei-hong², YANG Jia-hui², LI Jun^3*, LIU Xiao-Jun^1*

1. National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005;
2. Department of Microbiology and Immunology,College of Basic Medicine, Shanxi Medical University,Taiyuan 030001;
3. Department of Gastroenterology, Peking University Third Hospital, Beijing 100191, China

Received:2020-09-14 Revised:2020-10-28 Online:2021-02-05 Published:2021-01-19
Contact: ^*yanwt2003@163.com; xiaojunliu@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的探求青稞酥油饮食对小鼠肠道微生物分布及葡聚糖硫酸钠(DSS)诱导的小鼠慢性溃疡性结肠炎的影响。方法定制饮食,将标准鼠繁殖料(N鼠料)中的大豆油和玉米次粉替换为等比例的酥油和生青稞粉,以制成定制饲料(C鼠料)。将28只5周龄雄性小鼠随机分为4组,每组7只: 1)NW组:饲养以N鼠料和水;2)ND组:饲养以N鼠料和1.8% DSS;3)CW组:饲养以C鼠料和水;4)CD组:饲养以C鼠料和1.8% DSS。以7 d 1.8% DSS溶液之后换10 d蒸馏水为1个周期,共进行3个周期完成建模。匹拉米洞法测定小鼠粪便隐血进行DAI评分,选取结肠组织HE染色及粪便微生物16S rRNA高通量测序。结果青稞酥油饮食可提高正常小鼠肠道菌群丰度,改善肠道菌群组成,其中别样棒菌属增加。加入DSS诱导溃疡性结肠炎模型后,青稞酥油饮食组DAI评分显著增高(P＜0.05),组织学检测结肠黏膜糜烂溃疡且肠组织腺体排列混乱,小鼠肠道菌群丰度和多样性降低(P＜0.05),但梭菌属、萨特氏菌属增加(P＜0.05)。结论青稞酥油饮食可增加正常小鼠肠道菌群丰度,改善肠道菌群组成,但对于DSS诱导的慢性溃疡性结肠炎炎性反应无显著改善。

关键词: 青稞酥油饮食, DSS诱导溃疡性结肠炎, 16S rRNA高通量测序, 肠道菌群

Abstract: Objective To explore the effect of barley plus butter dietary on the symptoms of dextran sodium sulfate induced chronic ulcerative colitis and intestinal microbial distribution in mice. Methods In customized feed(C feed), soybean oil and corn meal in standard mice breeding feed (N feed) were replaced with equal proportion of ghee and raw barley powder.28 male mice were randomly divided into 4 groups(n=7,each group):NW group fed with N feed and water; ND group fed with N feed and 1.8% DSS; CW group fed with C feed and water; CD group fed with C feed and 1.8% DSS. Colitis model was established by three cycles of 10 days supply of distilled water after 7 days supply of 1.8% DSS solution. Finally, the effects were evaluated by DAI score, colon HE staining and 16S rRNA high-throughput sequencing of gut microbes. Results The abundance of bacteria was increased and the diversity of intestinal flora, such as allobaculum, was improved in wild type mice fed on the barley and butter dietary. However, when the mice were treated with DSS, the barley plus butter dietary group had high DAI score(P＜0.05), showed obvious intestinal ulcers and disorganized intestinal glands by histopathological microscopy, and decreased intestinal flora abundance and diversity(P＜0.05). But the quantity of Clostridium and Sartreella was increased in barley and butter dietary group(P＜0.05). Conclusions The barley plus butter dietary increases intestinal flora Allobaculum of wild type mice and improves the composition of intestinal flora, but the dietary intervention fails to show any significant impact on the chronic ulcerative colitis symptoms induced by DSS.

Key words: barley and butter dietary, DSS induced ulcerative colitis, 16S rRNA high-throughout sequencing, gut microbiota

中图分类号:

R574.1

李春美, 赵微, 解相宏, 张伟虹, 杨佳卉, 李军, 刘晓军. 青稞酥油饮食对小鼠肠道菌群以及葡聚糖硫酸钠诱导的小鼠结肠炎症状的影响[J]. 基础医学与临床, 2021, 41(2): 203-208.

LI Chun-mei, ZHAO Wei, XIE Xiang-hong, ZHANG Wei-hong, YANG Jia-hui, LI Jun, LIU Xiao-Jun. Effects of barley plus butter dietary on symptoms of intestinal flora in mice and dextran sodium sulfate induced ulcerative colitis[J]. Basic & Clinical Medicine, 2021, 41(2): 203-208.

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