关键词: EphrinAl, Caspase-3, T淋巴细胞, 重组腺病毒

Abstract: Objective Development of T cell strains infected by pAd-ephrinAl-caspase-3 recombinant adenovirus. Methods T lymphocytes were cultured . The constructed recombinant adenovirus infected T lymphocytes, Indirect immunofluorescence was used to detect the infection efficiency of T lymphocytes, The proliferation and toxicity of T lymphocytes were detected by MTT assay. The RT-PCR and ELISA were performed to detect expression of ephrinAl-caspase-3. Results T lymphocytes were successfully cultured and infected with recombinant adenovirus, the expression of ephrinAl and caspase3 increased. With the increase of MOI, the number of cell survival decreased and cell proliferation activity decreased（P<0.05）, and it showed time and dose dependence. Conclusion A T lymphocyte cell strain secreting ephrinA1-caspase-3 is successfully established.

Key words: EphrinAl, Caspase-3, T lymphocyte, recombinant adenovirus