关键词: miR-210, 肾透明细胞癌, 血管生成

Abstract: Objective To investigate the effect ofmiR-210 on angiogenesis of human renal clear cell carcinoma. Methods Detect the expression of miR-210 in 40 cases of renal clear cell carcinoma tissues, and analyze the relationship between the expression of miR-210 and microvessel density (MVD). MiR-210-ASO was lipotransfected into renal clear cell carcinoma cell line 786-O. RT-qPCR to verify the transfection effect. The effect of the supernatant of control group, negative control group and miR-210-ASO group tumor cells on the lumen formation of human umbilical vein endothelial cells (HUVEC) was observed in three dimensional culturesystems. The transplantation tumor model of nude mice was established, and the effect of miR-210 on the formation of the transplanted tumor micro vesselwas observed by endomucinand VEGF immunofluorescence staining under laser scanning confocal microscope. Results The expression of miR-210 was positively correlated with microvessel density in renal clear cell carcinoma (P<0.05).The expression of miR-210 was significantly decreased in 786-O cells transfected with miR-210-ASO(P<0.05). The lumen formation of HUVEC cells co cultured with miR-210-ASO group cell supernatant was significantly less than that of control group and negative control group(P<0.05). The tumor volume of miR-210-ASO group was less than that of the control group, and the number of the micro vesseland the VEGF expression were significantly less than that of the control group (P<0.05). Conclusions Inhibition of miR-210 can suppress the ability of blood vessel formation in renal clear cell carcinoma.

Key words: miR-210, renal clear cell carcinoma, Angiogenesis