Y-27632抑制胃癌SGC-7901细胞系侵袭与转移

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (10): 1369-1374.

Y-27632抑制胃癌SGC-7901细胞系侵袭与转移

赵敏,周颖,黄江梅,肖芳,李晓超,张辉,刘瑞吉

秦皇岛市第一医院

收稿日期:2015-03-13 修回日期:2015-05-23 出版日期:2015-10-05 发布日期:2015-09-30
通讯作者: 刘瑞吉 E-mail:zhaosg765@sohu.com

The effect of Y-27632 on invasion and migration of gastric carcinoma cell line SGC-7901

Received:2015-03-13 Revised:2015-05-23 Online:2015-10-05 Published:2015-09-30

摘要/Abstract

摘要： 目的探讨Y-27632对胃癌SGC-7901细胞侵袭和迁移的影响，及其作用机制是否是通过对SRF的调控来实现的。方法将细胞分为3组：1) 对照组；2) Y-27632通路阻断剂组；3) siRNA-SRF-1107干扰组。各组分别转染、加药，孵育48h。用CCK-8法检测细胞增殖，Transwell小室和划痕实验检测细胞侵袭能力和迁移能力。Western blot法检测SRF、ROCK1、 E-cadherin 、β-catenin、F-actin、MRTF-A 及 Cyclin D1的表达。结果 Y-27632能显著抑制胃癌SGC-7901细胞的侵袭、迁移能力（P<0.05），而对其增殖没有影响。Y-27632能显著下调SGC-7901细胞的ROCK1、SRF、F-actin和MRTF-A的表达，上调E-cadherin的表达（P<0.05）。结论 Y-27632通过上调E-cadherin表达，同时阻断ROCK信号抑制SRF辅因子MRTF-A的表达，最终下调SRF转录水平，从而抑制胃癌SGC-7901细胞侵袭、转移和迁移能力。

关键词: 胃癌, 血清应答因子（SRF）, Y-27632（ROCK通路阻断剂）, 胃癌SGC-7901细胞株, RNA干扰

Abstract: Objective To study the effect of Y-27632 on invasion and motility of SGC-7901 gastric carcinoma cells, and discuss whether Y-27632 excerts the effect by attenuating SRF expression. Methods SGC-7901 gastric carcinoma cells were divided into 3 groups: 1) blank control group; 2) Y-27632 group; 3) siRNA-SRF-1107 group. Transfected siRNA-SRF or incubated by Y-27632 48h. The effect of Y-27632 on proliferation suppressions of SGC-7901 gastric carcinoma cells was detected by CCK-8 assay. Cell invasion was analysed by Transwell and wound healing test. The expressions of SRF, ROCK1, E-cadherin, β-catenin, F-actin, MRTF-A and Cyclin D1 were detected by Western blot. Results Y-27632 inhibited invasion （P<0.05）and had no effect on proliferation of SGC-7901 gastric carcinoma cells（P>0.05）. Western blot showed that are treated with Y-27632, the ROCK1, MRTF-A, F-actin, SRF protein expressions were reduced by 37.0%, 44.3%, 62.7% and 62.7% respectively, and E-cadherin protein expression was up-regulated by 2.64 folds（P<0.05）.Conclusion The inhibition of ROCK and up-regulation of E-cadherin by Y-27632 can inhibit the invasion and migration of SGC-7901 gastric carcinoma cells that is done at least, in part, by attenuating SRF expression.

Key words: Gastric carcinoma, serum response factor(SRF), Y-27632(ROCK inhibitor), SGC-7901 cell line, RNA interference

赵敏周颖黄江梅肖芳李晓超张辉刘瑞吉. Y-27632抑制胃癌SGC-7901细胞系侵袭与转移[J]. 基础医学与临床, 2015, 35(10): 1369-1374.

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