关键词: 关键词：子宫内膜癌Ishikawa细胞, 光动力疗法, 细胞周期, 细胞凋亡

Abstract: Objective To investigate the effects of photodynamic therapy（PDT） on cell cycle and apoptosis in endometrial cancer Ishikawa cell line. Methods Ishikawa cell were treated by PDT with photosensitizer 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin(TMPyP). Cell morphology were observed by inverted microscope. Survival rate of Ishikawa cell was evaluated with Cell Counting Kit-8 (CCK-8). Cell cycle was analyzed by flow cytometry (FCM). Cell apoptosis was analyzed with Annexin V-FITC/PI Apoptosis Kit for FCM. The expression of Bcl-2、NF-κB P65 and phospho-NF-κB P65(p-NF-κB P65) and were detected by Western blotting. Results PDT could cause Ishikawa cell morphological changes. Survival rate decreased in Ishikawa cell treated by PDT after 24h（P＜0.05） and the rate was related with photosensitizer concentration and energy intensity. FCM analysis indicated PDT could induce S phase arrest and apoptosis（P＜0.05）. The expression of Bcl-2 protein and phosphorylation of NF-κB P65 were down regulated in Ishikawa cell after PDT（P＜0.05）. Conclusion PDT could induce S phase arrest and apoptosis in endometrial cancer Ishikawa cell , the possible mechanism is down-regulation of Bcl-2 and NF-κB.

Key words: Key words: endometrial cancer Ishikawa cell, photodynamic therapy, cell cycle, cell apoptosis