苦参碱通过下调SOCS3表达抑制支气管哮喘大鼠炎症反应并调节Th1/Th2平衡?

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (2): 191-195.

苦参碱通过下调SOCS3表达抑制支气管哮喘大鼠炎症反应并调节Th1/Th2平衡?

范临夏¹,潘辉¹,刘华²,蔡曦光³,陈其章¹,闫丽萍¹,王小军¹

1. 甘肃省人民医院
2. 甘肃省人民医院呼吸科
3. 兰州大学研究生院9号公寓324

收稿日期:2014-05-30 修回日期:2014-09-27 出版日期:2015-02-05 发布日期:2015-01-23
通讯作者: 刘华 E-mail:liuhua_lanzhou2@163.com
基金资助:
甘肃省卫生厅中医药项目

Matrine suppresses IFNlammation and correctes Th1/Th2 imbalance in asthmatic rats via downregulating SOCS3

Received:2014-05-30 Revised:2014-09-27 Online:2015-02-05 Published:2015-01-23

摘要/Abstract

摘要： 目的探讨苦参碱减轻支气管哮喘大鼠炎症反应、纠正Th1/Th2失衡的作用，以及SOCS3在此过程中的表达变化。方法将大鼠分为对照组、模型组（卵清蛋白致敏法构建大鼠急性哮喘模型）和药物干预A组及B组（建模成功后，分别接受60和120 mg/kg的苦参碱治疗）。计算支气管肺泡灌洗液（BALF）中的嗜酸性粒细胞（EOS）绝对值、EOS百分比及肺组织中的杯状细胞百分比和炎性细胞浸润积分。酶联免疫法分析BALF中的IFN-γ和IL-4水平，并计算IFN-γ/IL4。荧光实时定量PCR（qRT-PCR）和Western blot分析肺组织中细胞因子转导抑制因子3(SOCS3) mRNA与蛋白的表达。结果模型组大鼠BALF中EOS计数、EOS百分比、杯状细胞百分比、炎性细胞浸润评分和IL-4水平，肺组织中的SOCS3 mRNA及蛋白表达均较对照组显著增高（P <0.05）。而BALF中IFN-γ水平显著降低（P <0.05）。低剂量和高剂量苦参碱分别显著缓解模型组的上述变化（P <0.05）。结论苦参碱可以抑制支气管哮喘大鼠炎症反应、纠正Th1/Th2的失衡，该过程中存在SOCS3的表达变化。苦参碱可能通过降低SOCS3表达调节Th1/Th2的平衡。

关键词: 支气管哮喘, 苦参碱, IL-4, INF-γ, SOCS3, 大鼠

Abstract: Objective To investigate the inhibitory effect of matrine on IFNlammation by regulating Th1/Th2 balance in asthmatic rats and underlying mechanism related to SOCS3. Methods Rats, ovalbumin-sensitized to establish asthma model, SD rats were randomly divided into four groups, as follows: control (without any treatment), model group(using ovalbumin-sensitized method to establish asthma mode), treatment group A (low-dose matrine treated asthma rats) and treatment group B (high-dose matrine treated asthma rats). The eosinophil count, goblet cells percentage, IFNlammatory cell IFNiltration in rat lung were analyzed and scored by morphological examination. IL-4 and IFN-γ level in BALF were determined by ELISA and IFN-γ/IL-4 ratio was further calculated. Furthermore, the expression of SOCS3 in mRNA and protein level was detected by qRT-PCR and Western blot, respectively. Results Eosinophil count and percentage, goblet cell percentage and IFNlammatory cell IFNiltration score were significantly lower in treatment group A and B, compare to model group (P<0.05). Compared to control group, group A exhibited a lower IFN-γ level and a higher IL-4 level (P<0.05). IFN-γ level in treatment group A and B were higher while IL-4 level were lower, compare to model group. Meanwhile, SOCS3 mRNA level in rat lung tissue was elevated in model group, compare to control group. Matrine treatment decreased SOCS3 expression in treatment group A and B. Similar trend was found in SOCS3 protein level. Conclusion matrine may exhibit its anti-IFNlammatory effect by inhibiting SOCS3 expression and maintaining Th1/Th2 balance in asthmatic rats.

Key words: Asthma, Matrine, IL-4, INF-γ, SOCS3, Rats

范临夏潘辉刘华蔡曦光陈其章闫丽萍王小军. 苦参碱通过下调SOCS3表达抑制支气管哮喘大鼠炎症反应并调节Th1/Th2平衡?[J]. 基础医学与临床, 2015, 35(2): 191-195.

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