CCK-8降低LPS诱导的小鼠单核细胞RAW264.7 CHOP/caspase-11 /caspase-1的表达

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (2): 178-182.

CCK-8降低LPS诱导的小鼠单核细胞RAW264.7 CHOP/caspase-11 /caspase-1的表达

刘威¹,段林²,许光明³,于峰³,张晓静³,宋宁²

1. 河北省胸科医院
2. 河北医科大学第二医院
3. 河北医科大学

收稿日期:2014-05-12 修回日期:2014-10-13 出版日期:2015-02-05 发布日期:2015-01-23
通讯作者: 宋宁 E-mail:342840431@qq.com
基金资助:
CCK-8对巨噬细胞内质网应激启动炎症信号转导通路调节作用的研究

CCK-8 Decreases the Expressions of CHOP/Caspase-11/Caspase-1 Induced by LPS in Mouse Monocyte RAW264. 7 cell

Received:2014-05-12 Revised:2014-10-13 Online:2015-02-05 Published:2015-01-23

摘要/Abstract

摘要： 目的探讨CCK-8对LPS激活RAW264.7细胞CHOP/caspase-11/caspase-1 信号通路影响。方法用LPS和(或) CCK-8和(或) CCK-8的1受体阻滞剂孵育RAW 264. 7细胞，用western blot法检测CHOP、caspase-11蛋白表达，用试剂盒检测caspase-1活性，用ELISA技术检测细胞培养上清中IL-1β水平。结果与对照组相比，LPS组CHOP、caspase-11表达水平均显著升高(P﹤0.05)，给予CCK-8可有效抑制LPS诱导的CHOP、caspase-11表达升高(P﹤0.05)，而预先给予CCK-8的1受体阻滞剂可明显抑制CCK-8的作用。caspase-1活性变化、IL-1β含量变化趋势均与CHOP、caspase-11表达水平变化趋势一致(P﹤0.05)。结论 CCK-8通过受体1抑制CHOP/caspase-11/caspase-1表达，减少LPS诱导IL-1β的生成, 发挥抗炎作用。

关键词: 胆囊收缩素, 脂多糖, C/EBP同源蛋白, 半胱氨酸天冬氨酸蛋白酶11, 半胱氨酸天冬氨酸蛋白酶1

Abstract: Objective To investigate the effect of CCK-8 on CHOP/caspase-11/caspase-1 expressions by LPS in RAW264. 7 cell. Methods RAW264. 7 cells were incubated with LPS and/or CCK-8 and/or CCK-8 1R blocking agent devazepide for indicated times. CHOP and caspase-11 protein expressions were determined by western blot, caspase-1 activity were analyzed by kit, IL-1β expression levels were analyzed by ELISA. Results Both CHOP and caspase-11 expression were upregulated in LPS-activated RAW264. 7 cell, which were inhibited by pre-treatment of CCK-8. Pre-treatment of devazepide inhibited the effects of CCK-8 significantly.The same results were seen in caspase-1 activity and IL-1β expression. Conclusion CCK-8 exerts an anti-inflammatory effect by inhibiting CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264. 7 cell. CCK 1R might be responsible for the anti- inflammatory effect of CCK-8.

Key words: cholecystokinin, lipopolysaccharides, C/EBP homologous protein, caspase-11, caspase-1

中图分类号:

R563

刘威段林许光明于峰张晓静宋宁. CCK-8降低LPS诱导的小鼠单核细胞RAW264.7 CHOP/caspase-11 /caspase-1的表达[J]. 基础医学与临床, 2015, 35(2): 178-182.

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