关键词: 急性高原反应, 低氧诱导因子1, P38MAPK, 血管内皮生长因子, 缺氧

Abstract: Objective To explore the change of expression of hypoxia inducing factor 1(HIF-1? ), P38MARK and vascular endothelial growth factor (VEGF )after ascenting of plateau in vivo, and the correlation with the pathogenesy of AMS. Methods Collecting soldiers' venous blood before they asent of plateau and after they arrive in Yushu in Qinghai province 48 h later, extracting mononuclearcell the expression of mRNA of HIF-1? and P38MARK were detected by RT-PCR and the expression of protein of HIF-1? and VEGF was detected by Western blotting. The AMS is diagnosed according to《The Diagnose And Processing Principle Of AMS 》 Results After asentting of plateau the expression levels of mRNA of HIF-1? , P38MARK and the protein of HIF-1? and VEGF all increase significancely（p<0.01）. At the same time, the change of expression levels of HIF-1? and VEGF protein are highly corellated with AMS , the coefficient correlation is separately 0.806 and 0.675（p<0.001）. Conclusion The expression level of HIF-1?, P38MARK and VEGF increase significantly after asentting of plateau, especially the changes of HIF-1? protein and VEGF, it is highly correlated with the extent of AMS.

Key words: AMS, HIF-1α, P38MARK, VEGF, Hypoxia