基础医学与临床 ›› 2012, Vol. 32 ›› Issue (7): 778-782.

• 研究论文 • 上一篇    下一篇

AIDS患者γδ T 细胞能够杀伤自体HIV感染和未感染的CD4 T细胞

李珍1,焦艳梅2,3,崔莲仙4,吴昊2,何维5   

  1. 1. 中国医学科学院基础医学研究所
    2. 首都医科大学附属北京佑安医院
    3. 首都医科大学附属北京佑安医院感染科
    4. 医科院基础医学研究所
    5. 中国医学科学院基础医学研究所 北京协和医学院基础学院
  • 收稿日期:2012-05-03 修回日期:2012-05-24 出版日期:2012-07-05 发布日期:2012-06-20
  • 通讯作者: 何维 E-mail:heweiimu@public.bta.net.cn
  • 基金资助:
    国家重点基础研究发展规划(973计划);国家“十二五”重大专项

Lysis of autologous HIV-infected and uninfected CD4 T cells by γδ T cells from AIDS patients

  • Received:2012-05-03 Revised:2012-05-24 Online:2012-07-05 Published:2012-06-20
  • Contact: Wei HE E-mail:heweiimu@public.bta.net.cn

摘要: 目的:建立自体人类免疫缺陷病毒 (Human Immunodeficiency Virus, HIV) 感染的CD4 T细胞模型,检测获得性免疫缺陷综合症 (Acquired Immunodeficiency Syndrome, AIDS) 患者γδ T 细胞对自体HIV感染和未感染的CD4 T细胞的杀伤作用。方法:分离健康志愿者和未治疗的AIDS患者外周血单个核细胞 (PBMCs),磁珠分选获得高纯度的CD4 T细胞,加入植物血凝素(Phytohemagglutinin, PHA)和白细胞介素-2 (interleukin-2, IL-2) 共同培养,建立自体HIV感染的CD4 T细胞模型。流式细胞术检测HIV-p24+ CD4 T细胞的比例。乳酸脱氢酶 (Lactate dehydrogenase, LDH)法测定γδ T 细胞的细胞毒作用。结果:培养到第10 d时,HIV-p24+CD4 T细胞的比例达到最高值。AIDS患者的γδ T 细胞能够杀伤自体HIV感染和未感染的CD4 T细胞(细胞溶解率分别为71.1 ± 9.7和24.2 ± 18.2)。而健康志愿者γδ T 细胞对自体的CD4 T细胞没有杀伤作用(细胞溶解率为4.3 ± 10.1)。结论:AIDS患者γδ T 细胞能够杀伤自体HIV感染的CD4 T细胞,为采用γδ T 细胞过继免疫治疗AIDS提供了依据。

关键词: 关键词:γδ T 细胞, 人类免疫缺陷病毒, 获得性免疫缺陷综合症

Abstract: Objective: Establish a model of autologous Human Immunodeficiency Virus (HIV)-infected CD4 T cell, to investigate the cytotoxicity of γδ T cells of acquired immunodeficiency syndrome (AIDS) patients to autologous HIV-infected and uninfected CD4 T cells. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors and AIDS patients. CD4 T cells with high purity were obtained through negative isolation using magnetic activated cell sorting (MACS). To establish the autologous HIV-infected CD4 T cell model, CD4 T cells from AIDS patients were stimulated with phytohemagglutinin (PHA) and co-cultured with interleukin-2 (IL-2). Percentages of HIV-p24+ CD4 T cells were detected by flow cytometry. Cytotoxicity of γδ T cells was determined by Lactate dehydrogenase (LDH) assay. Results: The percentages of HIV-p24+ CD4 T cells reached the peak at 10 days. γδ T cells from AIDS patients can kill both the autologous HIV-infected and uninfected CD4 T cells (the cytolysis rate were 71.1 ± 9.7 and 24.2 ± 18.2,respectively). However, γδ T cells from healthy donors do not kill the autologous CD4 T cells (the cytolysis rate was 4.3 ± 10.1). Conclusions: γδ T cells of AIDS patients can kill the autologous HIV-infected CD4 T cells, which provide a new sight for the clinical therapy of AIDS based on γδ T cells adoptive immunotherapy.

Key words: Keywords: γδ T cells, HIV, AIDS

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