TGFβ1，CTGF信号转导通路的变化在实验性小鼠肝纤维发生中的作用

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (1): 66-70.

TGFβ1，CTGF信号转导通路的变化在实验性小鼠肝纤维发生中的作用

胡云龙¹,孔丽²,²

1. 河北医科大学第三医院
2.

收稿日期:2010-12-16 修回日期:2011-06-24 出版日期:2012-01-05 发布日期:2011-12-28
通讯作者: 胡云龙 E-mail:ydsyhyl@163.com

Mechanism of TGFβ1 and CTGF signaling pathway in experimental hepatic fibrosis

Received:2010-12-16 Revised:2011-06-24 Online:2012-01-05 Published:2011-12-28

摘要/Abstract

摘要： 摘要：目的探讨实验性肝纤维化小鼠肝组织中TGFβ1，CTGF信号转导通路的变化及意义。方法30只C57BL6/J小鼠随机分为正常对照组、肝纤维化模型组，采用10%的CCL4橄榄油腹腔注射诱导小鼠肝纤维化模型，对照组给予生理盐水灌胃，共造模8周。观察血清ALT、HA水平，HE染色、Masson染色观察肝组织炎症及纤维化程度，免疫组化法和RT-PCR法对肝组织α-SMA、TGFβ1、TGFβRⅡ、Smad3，Smad7，CTGF蛋白和mRNA水平进行检测，并与对照组肝组织进行比较。结果模型组小鼠血清ALT及HA水平明显高于对照组；模型组小鼠肝组织α-SMA、TGFβ1、TGFβRII、Smad3、CTGF蛋白表达和TGFβ1、Smad3、CTGF mRNA表达明显高于对照组，而模型组肝组织Smad7蛋白和Smad7 mRNA表达较对照组小鼠显著降低。结论 TGFβ1和CTGF信号转导通路过度活化，Smad7表达和负调节TGFβ、CTGF信号转导通路的功能被抑制可能与肝纤维化的发生和发展密切相关。

关键词: 关键词：肝纤维化, TGFβ1, Smad蛋白, CTGF, 信号转导通路

Abstract: Abstract Objective To investigate the mechanism of TGFβ1 and CTGF signaling pathway in experimental hepatic fibrosis mice. Methods 30 C57BL6/J mice were divided into control group and model group (each group, n=15). Mice were injected with CCL4 which induced liver fibrosis. Serum ALT and HA were tested by enzymic method and radio-immunity. Liver inflammation was graded by HE staining, and liver fibrosis by Masson staining. The expression of proteins of TGFβ1，CTGF，TGFβRII，Smad3，Smad7 of liver tissue were examined by immunohistochemistry. The expression of TGF-β1, Smad3，Smad7 and CTGF mRNA were analyzed by RT-PCR. Results Compared with control group, the expression of TGFβ1, CTGF，TGFβRII, Smad3 proteins and TGFβ1, CTGF，Smad3 mRNA were increased but Smad7 protein and Smad7 mRNA were decreased in model group. The levels of ALT and HA was higher in model group than control group. Conclusion TGFβ1 and CTGF pathway overactivitied may be associated with liver fibrosis. Down-regulating TGFβ, CTGF pathway and up-regulating smad7 expression may be suppressing the hepatic fibrosis.

Key words: Key words: Hepatic fibrosis, Smad protein, TGFβ1, CTGF, signalling pathway

胡云龙孔丽. TGFβ1，CTGF信号转导通路的变化在实验性小鼠肝纤维发生中的作用[J]. 基础医学与临床, 2012, 32(1): 66-70.

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