过表达miR-150的肾小管上皮细胞系HK-2中lncRNA的表达谱

doi:10.16352/j.issn.1001-6325.2022.07.1013

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (7): 1013-1019.doi: 10.16352/j.issn.1001-6325.2022.07.1013

• 研究论文 • 下一篇

过表达miR-150的肾小管上皮细胞系HK-2中lncRNA的表达谱

郝相楠¹, 栾军军¹, 马聪¹, 张旖骁², 周华^1*

中国医科大学附属盛京医院 1.肾内科; 2.泌尿外科, 辽宁沈阳 110004

收稿日期:2021-04-15 修回日期:2021-10-15 出版日期:2022-07-05 发布日期:2022-06-29
通讯作者: * huazhou_cmu@163.com
基金资助:
国家重点研发计划(2017YFC0907400);国家自然科学基金(81770698);辽宁省重点研发指导计划(2019JH8/10300009);辽宁省攀登学者(2013222)

Expression profile of lncRNA in miR-150 over-expressed cell line HK-2

HAO Xiang-nan¹, LUAN Jun-jun¹, MA Cong¹, ZHANG Yi-xiao², ZHOU Hua^1*

1. Department of Nephrology; 2. Department of Urology, the Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, China

Received:2021-04-15 Revised:2021-10-15 Online:2022-07-05 Published:2022-06-29
Contact: * huazhou_cmu@163.com

摘要/Abstract

摘要： 目的分析人肾小管上皮细胞系HK-2中miR-150过表达后长链非编码RNA(lncRNA)的差异表达,探究miR-150通过影响lncRNA的表达而致肾小管损伤的作用机制。方法采用Illumina NovaSeq 6000测序仪对过表达miR-150组和阴性对照组的HK-2细胞测序;对差异表达的lncRNA进行表达谱、GO、KEGG 分析;对其中的miR-150-lncRNA-JAK2-STAT1/3-COL1通路进行验证。结果和阴性对照组相比,过表达miR-150组共检测出362个差异表达的lncRNAs,其中252个lncRNAs显著下调,110个lncRNAs显著上调,差异均为1.5倍以上;通过GO富集分析发现miR-150通过lncRNA参与生物过程、细胞组分、分子功能;通过KEGG通路富集分析并验证发现了miR-150-lncRNA-JAK2-STAT1/3-COL1通路。结论 miR-150可通过lncRNAs在肾小管损伤的发生发展中发挥作用。

关键词: 肾小管上皮细胞, miR-150, 长链非编码RNA, 表达谱, HK-2

Abstract: Objective To analyze differential expression of long non-coding RNA (lncRNA) after miR-150 over-expression by human renal tubular epithelial cell line HK-2 and explore the role of miR-150 in renal tubular injury through regulating the expression of lncRNA. Methods HK-2 cells with miR-150 mimic and scramble were sequenced by Illumina NovaSeq 6000 sequencer. The differentially expressed lncRNA expression profile, GO analysis and KEGG analysis were performed to verify miR-150-lncRNA-JAK2-STAT1/3-COL1 pathway. Results Compared with the scramble group, 362 differential expression lncRNAs were detected in miR-150 mimic group, of which, 252 lncRNAs were down-regulated and 110 lncRNAs were up-regulated both more than 1.5-fold. The results showed that the miR-150 regulated lncRNA and function in biological process, cell components and molecular mechanisms through enrichment analysis of KEGG pathway, miR-150-lncRNA-JAK2-STAT1/3-COL1 pathway was found and then verified by Western blot. Conclusions LncRNAs regulated by miR-150 may play some roles in pathogenesis and molecular mechanisms in human renal tubular injury.

Key words: renal tubular epithelial cell, miR-150, long noncoding RNA, expression profile, HK-2

中图分类号:

R692

郝相楠, 栾军军, 马聪, 张旖骁, 周华. 过表达miR-150的肾小管上皮细胞系HK-2中lncRNA的表达谱[J]. 基础医学与临床, 2022, 42(7): 1013-1019.

HAO Xiang-nan, LUAN Jun-jun, MA Cong, ZHANG Yi-xiao, ZHOU Hua. Expression profile of lncRNA in miR-150 over-expressed cell line HK-2[J]. Basic & Clinical Medicine, 2022, 42(7): 1013-1019.

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过表达miR-150的肾小管上皮细胞系HK-2中lncRNA的表达谱

Expression profile of lncRNA in miR-150 over-expressed cell line HK-2

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