功能基因筛选和鉴定策略的研究进展及其优缺点

doi:10.16352/j.issn.1001-6325.2022.06.010

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (6): 969-974.doi: 10.16352/j.issn.1001-6325.2022.06.010

功能基因筛选和鉴定策略的研究进展及其优缺点

刘旭¹, 常德^1*, 王俊锋²

1.中国人民解放军总医院第三医学中心,北京 100069;
2.中国人民解放军总医院第二医学中心,北京 100853

收稿日期:2020-09-07 修回日期:2022-04-11 出版日期:2022-06-05 发布日期:2022-06-02
通讯作者: * changde5501@foxmail.com
基金资助:
国家十四五重点研发计划青年科学家项目(2021YFC2302300);解放军总医院青年科技专项(QNF19074);北京市科技新星计划交叉学科合作课题(Z191100001119021);后勤科研重点项目(BWS17J030)

Advantages, disadvantages and research progress on strategies for screening and identifying functional genes

LIU Xu¹, CHANG De^1*, WANG Jun-feng²

1. the Third Medical Center,Chinese People's Liberation Army General Hospital, Beijing 100069;
2. the Second Medical Center,Chinese People's Liberation Army General Hospital, Beijing 100853, China

Received:2020-09-07 Revised:2022-04-11 Online:2022-06-05 Published:2022-06-02
Contact: * changde5501@foxmail.com

摘要/Abstract

摘要： 筛选和鉴定功能基因是研究各种生物学表型发生分子机制的关键步骤,也是人类后基因组时代主要研究内容之一。传统遗传学、全基因组关联分析、表达谱差异、基因组测序、生物信息学等基于正向遗传学的筛选方法和cDNA文库、RNA干扰、CRISPR/Cas9技术、插入突变、模式生物等基于反向遗传学的筛选策略已被应用于筛选和鉴定功能基因,各种技术方法均具备各自的优势和缺陷,但可相互结合使用,发挥各自优点。

关键词: 功能基因, 正向遗传, 反向遗传, 生物信息学, 模式生物

Abstract: The screening and identification of functional genes is a key step to deeply understand various biological phenotypes. Forward genetics including traditional genetics, genome-wide association analysis, the comparison of gene expression profiles and bioinformatics, and reverse genetics including cDNA library, RNA interference, CRISPR-Cas9 technology, insertion mutation as well as model organisms have been applied to screening functional genes. Different methods have their own advantages and disadvantages and technology combination to screen and to identify functional genes is a good choice of strategy.

Key words: functional genes, forward genetics, reverse genetics, bioinformatics, model organism

中图分类号:

刘旭, 常德, 王俊锋. 功能基因筛选和鉴定策略的研究进展及其优缺点[J]. 基础医学与临床, 2022, 42(6): 969-974.

LIU Xu, CHANG De, WANG Jun-feng. Advantages, disadvantages and research progress on strategies for screening and identifying functional genes[J]. Basic & Clinical Medicine, 2022, 42(6): 969-974.

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功能基因筛选和鉴定策略的研究进展及其优缺点

Advantages, disadvantages and research progress on strategies for screening and identifying functional genes

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