兔颈动脉血流灌注减少促进血管内皮细胞炎性反应

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (9): 1247-1252.

• 研究论文 • 下一篇

兔颈动脉血流灌注减少促进血管内皮细胞炎性反应

生燕¹, 李秀毛², 郭欣¹, 谢仟¹, 黄仁杰¹, 生欣^3*

遵义医科大学基础医学院 1.形态学实验室; 3.生物化学教研室,贵州遵义 563000;
2.遵义医科大学附属医院胸心外科, 贵州遵义 563000

收稿日期:2020-07-01 修回日期:2021-01-15 出版日期:2021-09-05 发布日期:2021-09-02
通讯作者: *xshengbio@163.com
基金资助:
国家自然科学基金(31360278)

Reduction of carotid perfusion promotes inflammation of vascular endothelial cells in rabbits

SHENG Yan¹, LI Xiu-mao², GUO Xin¹, XIE Qian¹, HUANG Ren-jie¹, SHENG Xin^3*

1. Laboratory of Basic Medical Morphology; 3. Department of Biochemistry, School of Basic Medicine, Zunyi Medical University, Zunyi 563000;
2. Department of Cardiothoracic Surgery, the Affiliated Hospital of Zunyi Medical College, Zunyi 563000, China

Received:2020-07-01 Revised:2021-01-15 Online:2021-09-05 Published:2021-09-02
Contact: *xshengbio@163.com

摘要/Abstract

摘要： 目的探索动脉粥样硬化(AS)发生过程中,血流灌注减少与炎性反应之间的关系,及其对细胞骨架相关蛋白的调控作用。方法取新西兰兔,通过颈动脉套管建立了颈动脉狭窄模型(套管组),并以正常饲养与高脂饲养动物为对照组和高脂组。用免疫组化法结合qPCR和Western blot检测颈动脉中血管炎性反应相关蛋白MCP-1、平面极性信号通路相关蛋白DVL2与VANGL2、细胞骨架蛋白BBS8和γ-tubulin的组织定位和表达。结果高脂与套管组均可见内膜增生,且套管组更为严重。DVL2与VANGL2主要定位于血管内膜增生处,且两者在套管组的阳性定位均显著高于另外两组(P＜0.01)。此外,MCP-1等5种蛋白的表达在套管组中均显著上调(P＜0.05,P＜0.01)。结论颈动脉狭窄导致的血流灌注减少能够通过诱导血管MCP-1的上调参与AS发生早期的炎性反应,并通过DVL2与VANGL2调控微管组织中心蛋白BBS8和γ-tubulin调控微管类细胞骨架的重排。

关键词: 血流灌注, 动脉粥样硬化, 平面细胞极性, 单核细胞趋化蛋白1, 细胞骨架

Abstract: Objective To study how does reduction of blood flow perfusion regulates cytoskeleton rearrangement and its relationship with inflammation during atherosclerosis (AS). Methods The carotid artery stenosis model was established by carotid cannula in New Zealand rabbits. The normal animals were set as control group, and animals in high-fat diet were set as high-fat group. The localization and expression of vascular inflammation-related proteins MCP-1, planar cell polarity signaling pathway-related proteins DVL2 and VANGL2, and cytoskeletal proteins BBS8 and γ-tubulin were determined by immunohistochemistry staining,qPCR and Western blot. Results The cannula group and high-fat group but not control group showed intimal hyperplasia.The localizations of DVL2 and VANGL2 were found mainly at the endovascular hyperplasia in the cannula group, while the positive localizations of them were hardly found in the other two groups. Moreover, the expressions of the seproteins have been significantly up-regulated in the cannula group as compared to that in the control or high-fat group. Conclusions Reduction of blood flow perfusion is involved in the early inflammation of AS by increasing expression of MCP-1, and regulates the BBS8 and γ-tubulin during the rearrangement of microtubule cytoskeleton.

Key words: blood flow perfusion, atherosclerosis, planar cell polarity, monocyte chemotactic protein 1, cytoskeleton

中图分类号:

R318.01

生燕, 李秀毛, 郭欣, 谢仟, 黄仁杰, 生欣. 兔颈动脉血流灌注减少促进血管内皮细胞炎性反应[J]. 基础医学与临床, 2021, 41(9): 1247-1252.

SHENG Yan, LI Xiu-mao, GUO Xin, XIE Qian, HUANG Ren-jie, SHENG Xin. Reduction of carotid perfusion promotes inflammation of vascular endothelial cells in rabbits[J]. Basic & Clinical Medicine, 2021, 41(9): 1247-1252.

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兔颈动脉血流灌注减少促进血管内皮细胞炎性反应

Reduction of carotid perfusion promotes inflammation of vascular endothelial cells in rabbits

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