关键词: 胶质瘤, IDH1, 干扰素通路, IFIT3

Abstract: Objective To explore the role of IDH1 in the expression of RNA binding proteins (RBPs) in glioma cell line LN229. Methods Lentiviruses were used to construct IDH1 knockdown and control cells, and the expression of IDH1 was validated by RT-qPCR (Real-time quantitative PCR) and Western blot. Then the total RNA of cells from each group were extracted for RNA sequencing (RNA-seq); further, the differentially expressed genes (DEGs) were identified by limma and enriched pathways were analyzed; the differentially expressed RBPs were selected according to the RBP database and protein-protein interaction network was constructed by String database; finally, the expression level of candidate RBPs were tested in IDH1 knockdown, overexpressed and mutant cells respectively. Results A total of 259 DEGs were detected in IDH1 knockdown cells, including 146 upregulated and 113 downregulated genes. These DEGs were significantly enriched in interferon related pathways and included 14 RBP-encoding genes, 9 of which were significantly enriched in interferon related pathways and showed close interaction; compared with the control group, IDH1 knockdown in LN229 significantly upregulated the expression of all the nine RBPs (P < 0.001), and IDH1 overexpression only downregulated IFIT3 (P < 0.01) involving in interferon pathway, while IDH1 mutation had no significant effect on IFIT3. Conclusions IDH1 could regulate the expression of interferon pathway related gene IFIT3 in glioma cell line LN229.

Key words: glioma, IDH1, interferon pathway, IFIT3