基础医学与临床 ›› 2021, Vol. 41 ›› Issue (7): 982-987.

• 研究论文 • 上一篇    下一篇

多肽P7对肿瘤组织的靶向识别与Hsp90表达水平相关

孔德欣1,黄薇2,杨楠3,刘雁勇3   

  1. 1. 中国医学科学院基础医学研究所 北京协和医学院基础学院
    2. 广州医科大学附属第四医院
    3. 中国医学科学院基础医学研究所,北京协和医学院基础学院
  • 收稿日期:2021-04-14 修回日期:2021-05-19 出版日期:2021-07-05 发布日期:2021-06-17
  • 通讯作者: 刘雁勇 E-mail:yanyongliu@ibms.pumc.edu.cn
  • 基金资助:
    国家科技重大专项;国家自然科学基金;北京市自然科学基金;中国医学科学院医学与健康科技创新工程;协和青年科研基金与中央高校基本科研业务费专项基金

Correlation between the recognition of tumor targeting peptide P7 and Hsp90 expression level

  • Received:2021-04-14 Revised:2021-05-19 Online:2021-07-05 Published:2021-06-17
  • Contact: yong YanLIU E-mail:yanyongliu@ibms.pumc.edu.cn

摘要: 目的 分析热休克蛋白90(Hsp90)双功能靶向抑制多肽LPLTPLP(P7)对不同肿瘤组织及癌旁组织的识别效果,以及与热休克蛋白90(Hsp90)表达的相关性。方法 通过免疫组化和免疫荧光染色法分别检测组织芯片中48例16种器官肿瘤及匹配或不匹配的边缘或癌旁组织的Hsp90表达水平以及FITC-P7的识别效果,并对两组数据进行相关性分析。结果 在肿瘤组织中,Hsp90高表达且对FITC-P7的识别效果更好,两组数据呈现中度相关性(r=0.5147,P<0.01)。结论 P7对肿瘤组织的识别与其Hsp90表达水平相关,证实了P7靶向Hsp90的能力。

关键词: 热休克蛋白90, 多肽, 相关性分析

Abstract: Objective To analyze the correlation between the expression of heat shock protein 90 (Hsp90) and the recognition of LPLTPLP (P7) on different tumor tissues and para-carcinoma tissues. Method The expression levels of HSP90 and the recognition effects of FITC-P7 in 48 cases of 16 types of organ tumors and matched or mismatched marginal or adjacent tissues were analyzed by immunohistochemistry and immunofluorescence, respectively. Result Hsp90 was highly expressed in tumor tissues than para-carcinoma tissues. FITC-P7 showed a preferred recognition effect on tumor tissues in proportion to Hsp90 expression levels as determined by immunohistochemistry. There was moderate correlation the two group of data (r=0.5147,P<0.01). Conclusion The recognition of P7 to tumor tissues is positively related to the expression level of Hsp90, suggesting the ability of P7 to target Hsp90.

Key words: Hsp90, polypeptide, correlation analysis

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