关键词: 关键词：结节性硬化症, mTOR, 肿瘤微环境, 雷帕霉素

Abstract: Objective To explore the effect of sodium hydroxide and rapamycin alone or combination in treating Tsc2-/- cells. Methods The wild type and Tsc2-/- cells were treated with sodium hydroxide and/or rapamycin and divided into 4 groups: control, NaOH, rapamycin (Rapa), and combination (Combo) groups. Cell proliferation was measured by MTT assay and colony formation. Flow cytometry was used to detect the influence of cell cycle. The protein expression levels of S6, p-S6, p-4EBP1 were detected by Western Blot. Results Compared with control group, both Tsc2-/- cells in NaOH and Rapa groups demonstrated the reduced proliferation rate and colonies number (P <0.05); additionally, only in Rapa group, the percentage of Tsc2-/- cells in G1 phase increased and the protein levels of p-S6 and p-4EBP1 decreased (P <0.05). Compared with NaOH and Rapa groups, Tsc2-/- cells in Combo group had a further decreased in proliferation rate and colonies number, and the increased G1 phase percentage (P <0.05). But the p-S6 and p-4EBP1 levels of Tsc2-/- cells in Combo group were only significantly lower than those in NaOH group (P <0.05). Conclusion Sodium hydroxide could enhance the efficacy of rapamycin in suppressing Tsc2-/- cells proliferation.

Key words: Keywords: Tuberous sclerosis complex, mTOR, tumor microenvironment, Rapamycin