关键词: 泛素结合酶E2W, 葡聚糖硫酸钠盐, 结肠炎

Abstract: Objective To investigate the effect of UBE2W on dextran sodium sulfate (DSS) - induced ulceractive colitis in mice. Methods Different interference sequences of UBE2W siRNA (1#, 2# and 3#) were transiently transfected into mouse fibroblast L929. The expression of UBE2W protein was detected by Western blot. The siRNA with best inhibitory effect was selected for transfection in vivo. Ten C57BL / 6 male mice were randomly injected with siRNA (siUBE2W / siControl) via caudal vein and fed with 2.5% DSS to construct two groups of DSS colitis models, namely UBE2W knockdown group and control group with 5 mice in each group. Western blot and RT-qPCR were used to detect the expression of UBE2W in colon tissue of mice. The weight of mice in the two groups was observed, and the severity of colitis was compared by histological score. Results UBE2W siRNA 2# inhibited UBE2W protein expression most significantly in L929 cells. UBE2W mRNA and protein expression levels in colon tissue of UBE2W knockdown group were lower than those in control group, and weight loss in UBE2W knockdown group was significantly more than that in control group, and the histological score of colitis in knockdown group was 8.60 ± 0.24, which was higher than that in control group (5.20 ± 0.49, P < 0.05). Conclusion UBE2W is involved in the regulation of inflammatory response, UBE2W knockdown can increase the susceptibility of DSS induced colitis in mice.

Key words: ubiquitin-conjugating enzyme E2W, dextran sodium sulfate, colitis