基础医学与临床 ›› 2021, Vol. 41 ›› Issue (7): 941-945.

• 研究论文 • 上一篇    下一篇

白芨多糖上调溃疡性结肠炎小鼠肠黏膜紧密连接蛋白occludin的表达

李雨芯1,余雪嫣1,黄雪2   

  1. 1. 广西医科大学第一附属医院
    2. 广西医科大学第一附属医院老年病学消化科
  • 收稿日期:2021-01-07 修回日期:2021-05-19 出版日期:2021-07-05 发布日期:2021-06-17
  • 通讯作者: 黄雪 E-mail:hb960305@163.com
  • 基金资助:
    国家自然科学基金;广西研究生教育创新计划资助项目

Bletilla striata polysaccharide up-regulates the expression of tight junction protein occludin in intestinal mucosa of mice with ulcerative colitis

  • Received:2021-01-07 Revised:2021-05-19 Online:2021-07-05 Published:2021-06-17
  • Contact: Xue HUANG E-mail:hb960305@163.com

摘要: 目的 探讨白芨多糖(BSP)对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)小鼠的作用及其对肠黏膜紧密连接occludin蛋白的影响。方法 将小鼠随机分为对照组、DSS模型组(自由饮用2.5%DSS溶液7 d)、柳氮磺吡啶(300 mg/kg)阳性对照组以及BSP低(125 mg/kg)和高(250 mg/kg)剂量组。均从建模开始灌胃给药,1次/d,连续7 d。观察小鼠的体质量变化、疾病活动指数(DAI)评分、组织病理学评分及结肠长度等炎性反应,Western blot检测结肠组织occludin蛋白的表达。结果 与对照组比较,DSS模型组小鼠体质量显著减轻,结肠缩短,DAI评分和组织病理学评分显著升高(P<0.01),结肠组织occludin蛋白的表达显著减少(P<0.01)。与DSS模型组比较,BSP能够显著改善UC小鼠体质量减轻和结肠缩短,降低DAI评分和组织病理学评分,上调结肠组织occludin蛋白的表达。结论 BSP可以减轻DSS诱导的UC小鼠的结肠炎性反应,其作用机制可能与其上调肠黏膜occludin蛋白的表达,从而保护肠上皮屏障有关。

关键词: 白芨多糖, 溃疡性结肠炎, 肠上皮屏障, 紧密连接蛋白, occludin

Abstract: Objective To investigate the effect of Bletila striata polysaccharide on ulcerative colitis (UC) mice induced by Dextran sodium sulfate (DSS) and its effect on tight junction occludin protein in intestinal mucosa. Methods Mice were randomly divided into control group, DSS model group (freely drinking 2.5% DSS solution for 7 days), salazosulfopyridine(300 mg/kg) positive control group and BSP low-dose (125 mg/kg) and high-dose groups (250 mg/kg). From the beginning of modeling, Mice were given intragastric administration once a day for consecutive 7 days. The changes of body weight, disease activity index (DAI) score, histopathology score and colon length were observed to evaluate the inflammatory reaction. Expression of occludin protein in mice colon tissue were detected by Western blot. Results Compared with control group, the body weight of the model group mice was significantly reduced, the colon was shortened, the DAI and histopathology scores were significantly increased (P<0.01), and the expression of occludin protein in colon tissue was significantly reduced (P<0.01). Compared with DSS model group, BSP could significantly alleviate body weight loss and colon shortening, and decrease DAI and histological scores, and up-regulate the expression of occludin protein in colon tissue of UC mice. Conclusions BSP can alleviate the colonic mucosa inflammatory reactions in UC mice. The mechanism may be that it improves intestinal epithelial barrier function by increasing the expression of colonic mucosa occludin protein.

Key words: Bletilla striata polysaccharide, ulcerative colitis, intestinal epithelial barrier, tight junction protein, occludin

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