关键词: 白芨多糖, 溃疡性结肠炎, 肠上皮屏障, 紧密连接蛋白, occludin

Abstract: Objective To investigate the effect of Bletila striata polysaccharide on ulcerative colitis (UC) mice induced by Dextran sodium sulfate (DSS) and its effect on tight junction occludin protein in intestinal mucosa. Methods Mice were randomly divided into control group, DSS model group (freely drinking 2.5% DSS solution for 7 days), salazosulfopyridine(300 mg/kg) positive control group and BSP low-dose (125 mg/kg) and high-dose groups (250 mg/kg). From the beginning of modeling, Mice were given intragastric administration once a day for consecutive 7 days. The changes of body weight, disease activity index (DAI) score, histopathology score and colon length were observed to evaluate the inflammatory reaction. Expression of occludin protein in mice colon tissue were detected by Western blot. Results Compared with control group, the body weight of the model group mice was significantly reduced, the colon was shortened, the DAI and histopathology scores were significantly increased (P<0.01), and the expression of occludin protein in colon tissue was significantly reduced (P<0.01). Compared with DSS model group, BSP could significantly alleviate body weight loss and colon shortening, and decrease DAI and histological scores, and up-regulate the expression of occludin protein in colon tissue of UC mice. Conclusions BSP can alleviate the colonic mucosa inflammatory reactions in UC mice. The mechanism may be that it improves intestinal epithelial barrier function by increasing the expression of colonic mucosa occludin protein.

Key words: Bletilla striata polysaccharide, ulcerative colitis, intestinal epithelial barrier, tight junction protein, occludin