白芨多糖上调溃疡性结肠炎小鼠肠黏膜紧密连接蛋白occludin的表达

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (7): 941-945.

白芨多糖上调溃疡性结肠炎小鼠肠黏膜紧密连接蛋白occludin的表达

李雨芯, 余雪嫣, 黄雪^*

广西医科大学第一附属医院老年病学消化内科,广西南宁 530021

收稿日期:2021-01-07 修回日期:2021-05-19 出版日期:2021-07-05 发布日期:2021-06-17
通讯作者: *hb960305@163.com
基金资助:
国家自然科学基金(81660093);广西研究生教育创新计划(YCSW2020112)

Bletilla striata polysaccharide up-regulates the expression of tight junction protein occludin in intestinal mucosa of mice with ulcerative colitis

LI Yu-xin, YU Xue-yan, HUANG Xue^*

Department of Geriatrics and Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

Received:2021-01-07 Revised:2021-05-19 Online:2021-07-05 Published:2021-06-17
Contact: *hb960305@163.com

摘要/Abstract

摘要： 目的探讨白芨多糖(BSP)对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)小鼠的作用及其对肠黏膜紧密连接occludin蛋白的影响。方法将小鼠随机分为对照组、DSS模型组(自由饮用2.5%DSS溶液7 d)、柳氮磺吡啶(300 mg/kg)阳性对照组以及BSP低(125 mg/kg)和高(250 mg/kg)剂量组。均从建模开始灌胃给药,1次/d,连续7 d。观察小鼠的体质量变化、疾病活动指数(DAI)评分、组织病理学评分及结肠长度等炎性反应,Western blot检测结肠组织occludin蛋白的表达。结果与对照组比较,DSS模型组小鼠体质量显著减轻,结肠缩短,DAI评分和组织病理学评分显著升高(P＜0.01);结肠组织occludin蛋白的表达显著减少(P＜0.01)。与DSS模型组比较,BSP能够显著改善UC小鼠体质量减轻和结肠缩短,降低DAI评分和组织病理学评分,上调结肠组织occludin蛋白的表达。结论 BSP可以减轻DSS诱导的UC小鼠的结肠炎性反应,其作用机制可能与其上调肠黏膜occludin蛋白的表达,从而保护肠上皮屏障有关。

关键词: 白芨多糖, 溃疡性结肠炎, 肠上皮屏障, occludin

Abstract: Objective To investigate the effect of Bletila striata polysaccharide(BSP) on ulcerative colitis (UC) mice induced by dextran sodium sulfate (DSS) and its effect on tight junction occludin protein in intestinal mucosa. Methods Mice were randomly divided into control group, DSS model group (freely drinking 2.5% DSS solution for 7 days), salazosulfopyridine(300 mg/kg) positive control group, BSP low-dose (125 mg/kg) and high-dose groups (250 mg/kg). From the beginning of modeling, mice were given intragastric administration once a day for consecutive 7 days. The change of body weight, disease activity index (DAI) score, histopathology score and colon length were recorded to evaluate the inflammatory reaction. Expression of occludin protein in mice colon tissue was detected by Western blot. Results Compared with control group, the body weight of the model group mice was significantly reduced, the colon was shortened, the DAI and histopathology scores were significantly increased(P＜0.01), and the expression of occludin protein in colon tissue was significantly reduced (P＜0.01). Compared with DSS model group, BSP significantly alleviated body weight loss and colon shortening, and decrease DAI and histological scores, and up-regulated the expression of occludin protein in colon tissue of UC mice. Conclusions BSP alleviates inflammatory reactions of colonic mucosa in UC mice. The mechanism may be the improvement of intestinal epithelial barrier function mediated by increased expression of colonic mucosa occludin protein.

Key words: Bletilla striata polysaccharide, ulcerative colitis, intestinal epithelial barrier, occludin

中图分类号:

R574.62

李雨芯, 余雪嫣, 黄雪. 白芨多糖上调溃疡性结肠炎小鼠肠黏膜紧密连接蛋白occludin的表达[J]. 基础医学与临床, 2021, 41(7): 941-945.

LI Yu-xin, YU Xue-yan, HUANG Xue. Bletilla striata polysaccharide up-regulates the expression of tight junction protein occludin in intestinal mucosa of mice with ulcerative colitis[J]. Basic & Clinical Medicine, 2021, 41(7): 941-945.

参考文献

[1]Ng SC, Shi HY, Hamidi N, et al. Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: a systematic review of population-based studies[J]. Lancet, 2017,390:2769-2778.
[2]Luissint AC, Parkos CA, Nusrat A. Inflammation and the intestinal barrier: leukocyte-epithelial cell interactions, cell junction remodeling, and mucosal repair[J]. Gastroenterology, 2016,151:616-632.
[3]Lee SH. Intestinal permeability regulation by tight junction: implication on inflammatory bowel diseases[J]. Intest Res, 2015,13:11-18.
[4]Wang Y, Han S, Li R, et al. Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata[J]. Int J Biol Macromol, 2019,122:628-635.
[5]Diao H, Li X, Chen J, et al. Bletilla striata polysac-charide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages[J]. J Biosci Bioeng, 2008,105:85-89.
[6]黎笑兰, 张新广, 尹少萍. 白芨多糖抑制溃疡性结肠炎大鼠炎性反应与氧化应激[J]. 基础医学与临床, 2020,40:224-228.
[7]Hamamoto N, Maemura K, Hirata I, et al. Inhibition of dextran sulphate sodium (DSS)-induced colitis in mice by intracolonically administered antibodies against adhesion molecules (endothelial leucocyte adhesion molecule-1(ELAM-1) or intercellular adhesion molecule-1 (ICAM-1))[J]. Clin Exp Immunol, 1999,117:462-468.
[8]黄婧渝, 农辉, 裴羡, 等. 四君子汤对溃疡性结肠炎小鼠模型肠黏膜屏障的作用机制[J]. 世界华人消化杂志, 2015,23:4326-4334.
[9]柯昌毅, 赵春景. 白及多糖对溃疡性结肠炎的作用研究[J]. 中国药房, 2011,22:2132-2134.
[10]时松, 黄振, 罗熠, 等. 白芨多糖对小鼠溃疡性结肠炎的治疗作用[J]. 中国药科大学学报, 2012,43:535-540.
[11]Oshima T, Miwa H, Joh T. Changes in the expression of claudins in active ulcerative colitis[J]. J Gastroenterol Hepatol, 2008,23:S146-S150.
[12]Buckley A, Turner JR. Cell biology of tight junction barrier regulation and mucosal disease[J]. Cold Spring Harb Perspect Biol, 2018,10:a029314.doi:10.1101/cshperspect.a029314.
[13]Sakakibara A, Furuse M, Saitou M, et al. Possible involvement of phosphorylation of occludin in tight junction formation[J]. J Cell Biol, 1997,137:1393-1401.
[14]Al-Sadi R, Khatib K, Guo S, et al. Occludin regulates macromolecule flux across the intestinal epithelial tight junction barrier[J]. Am J Physiol Gastrointest Liver Physiol, 2011,300:G1054-G1064.