银屑病患者外周血淋巴细胞亚群的变化

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (3): 415-418.

银屑病患者外周血淋巴细胞亚群的变化

刘燕敏, 赵新程, 李俊琴, 张开明^*

山西医科大学附属太原中心医院皮肤科皮肤病学实验室免疫性皮肤病干细胞山西省重点实验室,山西太原 030009

收稿日期:2020-04-15 修回日期:2020-08-24 出版日期:2021-03-05 发布日期:2021-03-01
通讯作者: ^*zhangkaiming@sina.com
基金资助:
国家自然科学基金(8177333)

Changes of peripheral blood lymphocyte subsets in patients with psoriasis

LIU Yan-min, ZHAO Xin-cheng, LI Jun-qin, ZHANG Kai-ming^*

Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis, Dermatology Laboratry, Department of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan 030009, China

Received:2020-04-15 Revised:2020-08-24 Online:2021-03-05 Published:2021-03-01
Contact: ^*zhangkaiming@sina.com

摘要/Abstract

摘要： 目的探讨进行期、静止期和退行期的银屑病患者外周血淋巴细胞亚群的变化及免疫机制。方法本研究评估了77例寻常型银屑病患者(28例进行期、23例静止期、26例退行期)的外周血单个核细胞(PBMCs)各组中各淋巴细胞的比率。结果与退行期相比,进行期和静止期患者的CD3⁺、CD4⁺和CD8⁺淋巴细胞的百分率均显著升高(P＜0.01)。同进行期比较,静止期和退行期的CD4^-CD8^-淋巴细胞的百分率降低(P＜0.05)。CD3^-CD16⁺CD56⁺比例随病情稳定而增高,CD45⁺CD14⁺比例逐渐下降和CD3^-CD19⁺与病情呈负相关,3组的差异显著(P＜0.05)。结论进行期、静止期和退行期3组银屑病患者的淋巴细胞亚群存在显著的差异,进一步支持银屑病的免疫发病机制。

关键词: 银屑病, T淋巴细胞, 白细胞, B淋巴细胞

Abstract: Objective To investigate the changes and immune mechanisms of peripheral blood lymphocyte subsets in psoriasis patients with progressive, stationary and regression stages. Methods The percentage of lymphocytes in each group of peripheral blood mononuclear cells (PBMCs) in 77 patients with psoriasis vulgaris including 28 patients during progression, 23 patients during stationary, and 26 patients during regression. Results The percentages of CD3⁺, CD4⁺ and CD8⁺ lymphocytes were significantly increased in both the progression and stationary stages as compared to the regression stage (P＜0.01). CD4^- and CD8^- subsets in the peripheral blood lymphocyte subsets of the patients showed a decreasing trend according to progression, stationary and regression stages(P＜0.05). The result showed that the proportion of CD3^-CD16⁺CD56⁺ increased with the stability of the disease, the proportion of CD45⁺CD14⁺ gradually decreased, and the proportion of CD3^-CD19⁺ was negatively correlated with the disease (P＜0.05). Conclusions There are significant differences in lymphocyte subsets in the three groups of patients with psoriasis, which further supported the immune pathogenesis of psoriasis.

Key words: psoriasis, T lymphocyte, white blood cell, B lymphocyte

中图分类号:

刘燕敏, 赵新程, 李俊琴, 张开明. 银屑病患者外周血淋巴细胞亚群的变化[J]. 基础医学与临床, 2021, 41(3): 415-418.

LIU Yan-min, ZHAO Xin-cheng, LI Jun-qin, ZHANG Kai-ming. Changes of peripheral blood lymphocyte subsets in patients with psoriasis[J]. Basic & Clinical Medicine, 2021, 41(3): 415-418.

参考文献

[1] Pietrzak D, Pietrzak A, Krasowska D, et al. Digestive system in psoriasis: an update[J]. Arch Dermatol Res, 2017, 309:679-693.
[2] Stuart PE, Nair RP, Tsoi LC, et al. Genome-wide association analysis of psoriatic arthritis and cutaneous psoriasis reveals differences in their genetic architecture[J]. Am J Hum Genet, 2015, 97:816-836.
[3] Phan C, Touvier M, Kesse-Guyot E, et al. Association between mediterranean anti-inflammatory dietary profile and severity of psoriasis: results from the nutrinet-santé cohort[J]. JAMA Dermatol, 2018, 154:1017-1024.
[4] Rendon A, Schäkel K. Psoriasis pathogenesis and treatment[J]. Int J Mol Sci, 2019, 20:1475.doi:10.3390/ijms20061475.
[5] Woo YR, Cho DH, Park HJ. Molecular mechanisms and management of a cutaneous inflammatory disorder: psoriasis[J]. Int J Mol Sci, 2017, 18:E2684. doi:10.3390/ijms18122684.
[6] 陈曦, 张凯辉, 安军艳, 等. 寻常型银屑病患者三大基本中医证型外周血Th细胞分化与JAK/STAT信号通路的关[J]. 中华中医药杂志, 2019, 34:5964-5967.
[7] 姚梦, 刘鹏, 蒲凤, 等. T淋巴细胞亚群分析在免疫缺陷病患者中的应用及临床意义[J]. 卫生职业教育, 2020, 38:155-157.
[8] Prinz JC. Human leukocyte antigen-class Ⅰ alleles and the autoreactive T cell response in psoriasis pathogenesis[J]. Front Immunol, 2018, 9:954. doi:10.3389/fimmu.2018.00954.
[9] 缪旭, 范向华, 林桦, 等. 银屑病患者外周血CD16+ CD56+NK细胞含量与临床严重性相关性分析[J]. 中国麻风皮肤病杂志, 2013, 29:6-7.
[10] Vandenhaute J, Wouters CH, Matthys P. Natural killer cells in systemic autoinflammatory diseases: a focus on systemic juvenile idiopathic arthritis and macrophage activation syndrome[J]. Front Immunol, 2020, 10:3089. doi:10.3389/fimmu.2019.03089.
[11] Hu SC, Yu HS, Yen FL, et al. Neutrophil extracellular trap formation is increased in psoriasis and induces human β-defensin-2 production in epidermal keratinocytes[J]. Sci Rep, 2016, 6:31119. doi:10.1038/srep31119.
[12] 宋翠豪, 王睿, 赵梓纲, 等. 中性粒细胞和淋巴细胞比值与银屑病的相关性研究[J]. 实用皮肤病学杂志, 2019, 12:267-272.
[13] Lu J, Ding Y, Yi X, et al. CD19⁺ B cell subsets in the peripheral blood and skin lesions of psoriasis patients and their correlations with disease severity[J]. Braz J Med Biol Res, 2016, 49:e5374. doi:10.1590/1414-431X20165374.
[14] Taher TE, Bystrom J, Ong VH, et al. Intracellular B lymphocyte signalling and the regulation of humoral immunity and autoimmunity[J]. Clin Rev Allergy Immunol, 2017,53:237-264.

[1]	李钊璇, 罗云萍, 陈翀. 气液交互法培养小鼠来源的肿瘤类器官的建立及优化[J]. 基础医学与临床, 2022, 42(5): 782-787.
[2]	陈号, 陈韬, 吴静. IL-6/JAK/STAT3信号通路在前列腺癌发展和治疗中的研究进展[J]. 基础医学与临床, 2022, 42(1): 159-162.
[3]	宋茜黄雪覃蒙斌张君红井洁罗毅华李雨芯. STAT3通路参与白细胞介素-22促进人结肠癌细胞系HT29紧密连接蛋白的表达[J]. 基础医学与临床, 2021, 41(6): 792-798.
[4]	玛依努·玉苏甫, 衣巴地古丽·库吐鲁克, 哈尼克孜·阿不都艾尼. α-硫辛酸对自身免疫性甲状腺炎小鼠炎性反应的抑制作用[J]. 基础医学与临床, 2021, 41(1): 72-76.
[5]	刘佳奕, 章正祥, 严民力, 戚观树, 陈强, 侯群. 急性缺血性卒中患者血栓成分和影像学表现的研究进展[J]. 基础医学与临床, 2021, 41(1): 112-115.
[6]	孙慧颖, 朱崇元, 冯国栋, 高志强. 含有白细胞的富血小板纤维蛋白的生物学特性及临床应用[J]. 基础医学与临床, 2020, 40(12): 1706-1710.
[7]	陈思行王鸥聂敏姜艳李梅夏维波邢小平. 非手术性甲状旁腺功能减退症患者T淋巴细胞亚群的改变[J]. 基础医学与临床, 2019, 39(8): 1114-1118.
[8]	张乃月朱榕嘉尹秀平王巧玲韩钦赵春华宋坪. 射干苷和桃叶珊瑚苷抑制LPS诱导的人脂肪间充质干细胞炎性反应[J]. 基础医学与临床, 2019, 39(7): 961-966.
[9]	刘超高慧婕朱玉贞董晓磊崔巍王北. 大黄素对小鼠的免疫调节作用及对脾细胞TNF-α和IL-10表达的影响 [J]. 基础医学与临床, 2018, 38(9): 1298-1302.
[10]	钟根深尤朵陈亚楠杨如许芝山吴敏娜. 过表达IL-18的T细胞对胰腺癌细胞SW-1990的杀伤作用[J]. 基础医学与临床, 2017, 37(9): 1251-1256.
[11]	王如岭吴永娜王黎明李莹严祥. 肿瘤过继性细胞免疫治疗的研究进展[J]. 基础医学与临床, 2017, 37(7): 1055-1058.
[12]	王淑莉陈慧陆必超张建民何维. 添加细胞因子优化人外周血γδ T细胞的体外扩增培养体系[J]. 基础医学与临床, 2017, 37(6): 752-757.
[13]	张燕熊霞. IL-37及其与疾病相关性的研究进展[J]. 基础医学与临床, 2015, 35(4): 549-553.
[14]	崔金霞张永东时莹庆冯喜英王晋关巍. 高海拔COPD大鼠血浆TNF-α、IL-6和IL-10水平升高[J]. 基础医学与临床, 2014, 34(8): 1108-1109.
[15]	李壮董晨辉徐祥何静黄宏郭韡邢伟王爱民. 自体及健康人CIK细胞治疗临床恶性肿瘤的比较[J]. 基础医学与临床, 2014, 34(4): 475-479.