关键词: 槐果碱, 低氧, H9c2细胞, 缺血性心脏病

Abstract: Objective To investigate the inhibitory effect and mechanism of sophoridine on cell injury caused by hypoxia in rat myocardial cell line H9c2. Methods H9c2 cells were exposed to 1%O2 and cultured with different concentrations (1, 4 and 16 mmol/L) of sophoridine for 48 h. Cell viability was measured by MTT assay. The release level of LDH and the content of MDA were determined by the kit methods. Cell apoptosis was detected by flow cytometry. The protein expressions of cleaved caspase-3, p-PI3K and p-AKT were detected by Western blot. Results Sophoridine alleviated the cell viability, LDH release, MDA content and hypoxia-induced apoptosis of H9c2 cells in a concentration-dependent manner (P<0.05, P<0.01, P<0.001). Sophoridine decreased the cleaved caspase-3 expression and increased the ratios of p-PI3K/PI3K and p-AKT/AKT in a concentration-dependent manner (P<0.05, P<0.01, P<0.001). Conclusions Sophoridine can reduce the hypoxia-induced cell injury and has a protective effect on H9c2 cells, which may be achieved by enhancing the activity of PI3K/AKT signal.

Key words: Sophoridine, hypoxia, H9c2 cells, ischemic heart disease