基础医学与临床 ›› 2021, Vol. 41 ›› Issue (3): 382-387.

• 研究论文 • 上一篇    下一篇

槐果碱减轻低氧导致的大鼠心肌细胞系H9c2损伤

卢厚新1,杨清泉1,郭石2   

  1. 1. 河南省郑州大学附属医院南阳市中心医院
    2. 开封市儿童医院药学部
  • 收稿日期:2020-04-27 修回日期:2020-07-24 出版日期:2021-03-05 发布日期:2021-03-01
  • 通讯作者: 卢厚新 E-mail:15165305593@139.com

Sophoridine alleviates hypoxia-induced injury of rat myocardial cell line H9c2

  • Received:2020-04-27 Revised:2020-07-24 Online:2021-03-05 Published:2021-03-01

摘要: 目的 阐明槐果碱对低氧导致的大鼠心肌细胞系H9c2损伤的抑制作用及机制。方法 将H9c2细胞暴露于1%O2环境中,同时加入不同浓度(1、4和16 mmol/L)的槐果碱培养细胞48 h后,用MTT法测定细胞活力;用试剂盒分别检测LDH释放水平和MDA含量;用流式细胞仪检测细胞凋亡;用Western blot检测细胞中cleaved-caspase-3、p-PI3K和p-AKT的蛋白表达。结果 槐果碱呈浓度依赖性减轻由低氧导致的H9c2细胞的细胞活力降低、LDH释放、MDA含量增高和细胞凋亡(P<0.05,P<0.01,P<0.001)。槐果碱呈浓度依赖性降低cleaved caspase-3的表达(P<0.05,P<0.01,P<0.001),同时增加p-PI3K/PI3K和p-AKT/AKT的比率(P<0.05,P<0.01,P<0.001)。结论 槐果碱能减轻低氧导致的细胞损伤,对H9c2细胞发挥保护作用,且这一作用可能是通过增强PI3K/AKT信号活性来实现的。

关键词: 槐果碱, 低氧, H9c2细胞, 缺血性心脏病

Abstract: Objective To investigate the inhibitory effect and mechanism of sophoridine on cell injury caused by hypoxia in rat myocardial cell line H9c2. Methods H9c2 cells were exposed to 1%O2 and cultured with different concentrations (1, 4 and 16 mmol/L) of sophoridine for 48 h. Cell viability was measured by MTT assay. The release level of LDH and the content of MDA were determined by the kit methods. Cell apoptosis was detected by flow cytometry. The protein expressions of cleaved caspase-3, p-PI3K and p-AKT were detected by Western blot. Results Sophoridine alleviated the cell viability, LDH release, MDA content and hypoxia-induced apoptosis of H9c2 cells in a concentration-dependent manner (P<0.05, P<0.01, P<0.001). Sophoridine decreased the cleaved caspase-3 expression and increased the ratios of p-PI3K/PI3K and p-AKT/AKT in a concentration-dependent manner (P<0.05, P<0.01, P<0.001). Conclusions Sophoridine can reduce the hypoxia-induced cell injury and has a protective effect on H9c2 cells, which may be achieved by enhancing the activity of PI3K/AKT signal.

Key words: Sophoridine, hypoxia, H9c2 cells, ischemic heart disease