基础医学与临床 ›› 2021, Vol. 41 ›› Issue (3): 370-375.

• 研究论文 • 上一篇    下一篇

奥美拉唑治疗对反流性食管炎模型大鼠食管菌群构成的影响

高峰1,贾梦真2,孙玥1   

  1. 1. 首都医科大学附属北京安贞医院
    2. 北京市顺义区医院
  • 收稿日期:2020-06-04 修回日期:2020-07-07 出版日期:2021-03-05 发布日期:2021-03-01
  • 通讯作者: 高峰 E-mail:gaofeng4321@sina.com
  • 基金资助:
    北京市医院管理中心科研培育计划中医项目

Effects of administration of omeprazole on the esophageal microbiota composition in rat model with reflux esophagitis

  • Received:2020-06-04 Revised:2020-07-07 Online:2021-03-05 Published:2021-03-01
  • Contact: 高 峰 E-mail:gaofeng4321@sina.com
  • Supported by:
    Beijing Hospitals Authority Incubating Program

摘要: 目的 探讨质子泵抑制剂治疗对反流性食管炎大鼠食管黏膜菌群构成的影响。方法 采用幽门部分结扎及前胃完全结扎手术建立反流性食管炎大鼠模型。SD 大鼠随机分为假手术对照组、反流性食管炎对照组和质子泵抑制剂奥美拉唑治疗组,每组6只;术后1周开始灌胃,灌胃2 周后处死大鼠获取食管黏膜组织2块,1块用于细菌16S rDNA基因测序,1块用于病理检查。结果 假手术对照组、反流性食管炎对照组和质子泵抑制剂治疗组食管黏膜的属水平菌群构成检测如下:消化链球菌属丰度值分别为2.5%±0.5%,1.7%±0.7%和1.9%±0.5%;螺杆菌属丰度值分别为1.5%±0.1%,1.0%±0.5%和1.2%±0.3%;苏黎世杆菌属丰度值分别为1.0%±0.2%,0.5%±0.2%和1.0%±0.4%(P < 0.05);反流性食管炎对照组的以上三组菌属丰度值明显低于假手术对照组(P < 0.05)。反流性食管炎对照组和质子泵抑制剂治疗组病理分级(轻/中/重)分别为0/4/2和5/1/0(P < 0.05)。结论 反流性食管炎大鼠食管黏膜菌群构成发生改变,有益菌减少;灌服质子泵抑制剂奥美拉唑治疗后,食管黏膜损伤减轻,有益菌增加,菌群构成恢复正常。

关键词: 反流性食管炎, 质子泵抑制剂, 高通量DNA测序技术, 食管黏膜菌群构成

Abstract: Objective To investigate the effect of proton pump inhibitor (PPI) omeprazole treatment on the composition of esophageal mucosal microbiota in rats with reflux esophagitis. Methods The rat model of reflux esophagitis was established by the method of pyloric partial ligation and complete anterior gastric ligation. Rats were randomly divided into sham operation control group, reflux esophagitis control group and PPI treatment group (omeprazole). One week after the operation, distilled water / omeprazole tablets were intragastric administration once a day, and two pieces of lower esophageal mucosa were obtained 2 weeks later, one for bacteria 16S rDNA gene sequencing, and another for pathological examination. Results Six surviving rats in each group were selected. The abundance values of sham operation control, reflux esophagitis control and PPI treatment groups at the genus level were as follows: Peptostreptococcus 2.5%±0.5%, 1.7%±0.7% and 1.9%±0.5%; Helicobacter 1.5%±0.1%, 1.0%±0.5% and 1.2%±0.3%;Turicibacter 1.0%±0.2%, 0.5%±0.2% and 1.0%±0.4% (P < 0.05). The abundances of Peptostreptococcus, Helicobacter, and Turicibacter in the reflux esophagitis control group were significantly reduced than those in the sham operation control group. The pathological grades of reflux esophagitis (mild / moderate / severe) in reflux esophagitis control and PPI treatment groups were 0/4/2 and 5/1/0 (P < 0.05). Conclusion There were moderate changes in the composition of esophageal mucosal microbiota in rats with reflux esophagitis,the proportion of beneficial bacteria reduced. After the treatment of PPI omeprazole, the esophageal mucosal injury was alleviated, the proportion of beneficial bacteria increased, and the composition of esophageal mucosal microbiota returned to normal.

Key words: reflux esophagitis, proton pump inhibitor, high-throughput DNA sequencing, esophageal mucosal microbiotia

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