液相芯片技术与实时荧光定量PCR在检测儿童感染性腹泻病原体的比较

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (4): 523-527.

液相芯片技术与实时荧光定量PCR在检测儿童感染性腹泻病原体的比较

汉聪慧¹, 曹广进², 张福真¹, 胡亮杉³, 曹东林^3*, 李凌^1*

1.南方医科大学公共卫生学院三级生物安全实验室,广东广州 510515;
2.广州市番禺区疾病预防控制中心,广东广州 511400;
3.广东省第二人民医院临床检验中心, 广东广州 510317

收稿日期:2019-11-12 修回日期:2020-01-15 出版日期:2020-04-05 发布日期:2020-04-06
通讯作者: *liling@smu.edu.cn; caodl@126.com
基金资助:
2013年度广东省级财政技术研究开发与推广应用专项资金项目(粤财工[2013]401)

Comparison of liquid-phase chip and real-time fluorescent quantitative PCR for the detection of infectious diarrhoeal pathogens in children

HAN Cong-hui¹, CAO Guang-jin², ZHANG Fu-zhen¹, HU Liang-shan³, CAO Dong-lin^3*, LI Ling^1*

1. Biosafety Level 3 Laboratory, School of Public Health, Southern Medical University, Guangzhou 510515;
2. Panyu District Center for Disease Control and Prevention, Guangzhou 511400;
3. Department of Laboratory Medicine, Guangdong Second Provincial General Hospital, Guangzhou 510317, China

Received:2019-11-12 Revised:2020-01-15 Online:2020-04-05 Published:2020-04-06
Contact: *liling@smu.edu.cn; caodl@126.com

摘要/Abstract

摘要： 目的研究液相芯片检测儿童感染性腹泻病原体的效率,为感染性腹泻病的临床快速诊断提供依据。方法收集96例婴幼儿及儿童腹泻患者粪便样本,运用Luminex液相芯片进行检测,并与实时荧光定量PCR检测结果比较。结果液相芯片技术共检测出7种病原体,阳性检出率为65.63%(63/96),其中沙门氏菌的检出率最高,为50.00%(48/96);单病原感染的阳性率为39.58%(38/96),混合感染的阳性率为26.04%(25/96)。与实时荧光定量PCR相比,液相芯片检测腹泻病原体的敏感度和特异度分别为100.00%和71.74%,阴性预测值和阳性预测值分别为100.00%和79.37%(P＜0.05)。结论与实时荧光定量PCR相比,液相芯片技术具有高通量、快速、可靠等特点,有利于感染性腹泻病原体的临床检测。

关键词: 液相芯片, 实时荧光定量PCR, 感染性腹泻病, 病原体检测

Abstract: Objective To investigate the efficiency of liquid-phase chip on the detection of infectious diarrhea pathogens in children, and provide reference for rapid clinical diagnosis of infectious diarrhea diseases. Methods Stool samples of 96 infants and children with diarrhea were collected and detected by xTAG®Gastrointestinal Pathogen Panel (GPP), and the results were compared with the real-time PCR method. Results Total of 7 pathogens were detected by liquid-phase chip technology, with a positive detection rate of 65.63%(63/96), and the Salmonella detection rate was highest as 50%(48/96). The positive rate of single pathogen infection was 39.58%(38/96), and the positive rate of mixed infection was 26.04%(25/96). Overall, compared with the results of real-time PCR, the sensitivity and specificity of the liquid-phase chip in the detection of diarrhea pathogens were 100% and 71.74%, respectively, and negative predictive value and positive predictive value were 100.00% and 79.37%, respectively(P＜0.05). Conclusions Compared with real-time PCR, liquid-phase chip is a character-ized by high throughput, rapid and reliable so support laboratory diagnosis of contagious diarrhea

Key words: liquid-phase chip, real-time PCR, infectious diarrhea diseases, pathogen detection

中图分类号:

R331

汉聪慧, 曹广进, 张福真, 胡亮杉, 曹东林, 李凌. 液相芯片技术与实时荧光定量PCR在检测儿童感染性腹泻病原体的比较[J]. 基础医学与临床, 2020, 40(4): 523-527.

HAN Cong-hui, CAO Guang-jin, ZHANG Fu-zhen, HU Liang-shan, CAO Dong-lin, LI Ling. Comparison of liquid-phase chip and real-time fluorescent quantitative PCR for the detection of infectious diarrhoeal pathogens in children[J]. Basic & Clinical Medicine, 2020, 40(4): 523-527.

参考文献

[1]梁丹, 车荣飞, 石倩萍, 等. 感染性腹泻疾病负担及其病原谱变化的研究进展[J].中国公共卫生, 2019, 35: 467-470.
[2]GBD 2016 Disease and Injury Incidence and Prevalence Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016[J]. Lancet, 2017, 390: 121-159.
[3]GBD 2017 Causes of Death Collaborators. Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980-2017: a systematic analysis for the Global Burden of Disease Study, 2017[J]. Lancet, 2018, 392: 1736-1788.
[4]Chrisra L, Fischer W, Li Liu, et al. Global burden of childhood pneumonia and dierrbea[J]. Lancet, 2013, 381: 1405-1416.
[5]Li LL, Liu N, Humphries EM, et al. Aetiology of diarrhoeal disease and evaluation of viral-bacterial coinfection in children under 5 years old in China: a matched case-control study[J]. Clin Microbiol Infect, 2016, 22: 9-16.
[6]闫俊平, 曹东林. 液相芯片技术在传染病快速诊断中的应用[J]. 现代医院, 2017, 17: 1782-1785.
[7]曹广进. 利用液相芯片技术检测儿童急性呼吸道感染及感染性腹泻病原体的研究[D]. 广州: 南方医科大学, 2019: 34-79.
[8]Kotloff KL, Platts-Mills JA, Nasrin D, et al. Global burden of diarrheal diseases among children in developing countries:Incidence, etiology, and insights from new molecular diagnostic techniques[J]. Vaccine, 2017, 35: 6783-6789.
[9]Tickell KD, Brander RL, Atlas HE, et al. Identification and management of Shigella infection in children with diarrhoea: a systematic review and meta-analysis[J]. Lancet Glob Health, 2017, 5: 1235-1248.
[10]黄恩妙, 王翠玲, 王曼, 等. 2015-2016年中山市感染性腹泻病原学监测结果分析[J].现代预防医学, 2017, 44: 3040-3047.
[11]Casañ C, Ocete MD, Medina R, et al. Evaluation of the Luminex xTAG®GPP (Gastrointestinal Pathogen Panel) in the diagnosis of diseases with acute diarrhoea[J]. Enferm Infecc Microbiol Clin, 2017, 35: 574-577.
[12]Porte L, Varela C, Haecker T, et al. Impact of changing from staining to culture techniques on detection rates of Campylobacter spp. In routine stool samples in Chile[J]. BMC Infect Dis, 2016, 16: 196-202.
[13]Deng J, Luo X, Wang R, et al. A comparison of Luminex xTAG®Gastrointestinal Pathogen Panel (xTAG GPP) and routine tests for the detection of enteropathogens circulating in Southern China[J]. Diagn Microbiol Infect Dis, 2015, 83: 325-330.
[14]林丽娟, 彭雅冰, 罗欣, 等. 悬浮芯片法调查广州腹泻病原体分布及临床特征分析[J]. 实用医学杂志, 2016, 32: 3724-3727.