革兰阴性菌中分子伴侣在双组分蛋白分泌至周质空间过程中的作用

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (4): 456-459.

革兰阴性菌中分子伴侣在双组分蛋白分泌至周质空间过程中的作用

张艳娟, 王哲, 蔡锟, 黄丕英, 储引娣, 范恩国^*

中国医学科学院基础医学研究所北京协和医学院基础学院病原生物学系, 北京 100005

收稿日期:2019-12-27 修回日期:2020-01-10 出版日期:2020-04-05 发布日期:2020-04-06
通讯作者: *enguo.fan@ibms.pumc.edu.cn
基金资助:
国家自然科学基金(31770901);中国医学科学院医学与健康科技创新工程(CAMS-I2M-2017);北京协和医学院“中央高校基本科研业务费”(3332019065)

Function of molecular chaperones in the secretion of two-partner-secretion to the periplasm in Gram-negative bacteria

ZHANG Yan-juan, WANG Zhe, CAI Kun, Huang Pi-ying, CHU Yin-di, FAN En-guo^*

Department of Microbiology and Parasitology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/ College of Basic Medicine, Peking Union Medical College, Beijing 100005, China

Received:2019-12-27 Revised:2020-01-10 Online:2020-04-05 Published:2020-04-06
Contact: *enguo.fan@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的探究革兰阴性菌中分子伴侣在双组分蛋白跨细菌内膜及周质空间中的作用。方法以具有代表性的双组分蛋白FhaB^*/FhaC系统为例,使用不同分子伴侣蛋白的敲除菌株,制备成原生质球后,研究不同的分子伴侣对底物蛋白FhaB^*分泌至周质空间的影响。结果与野生型相比,分子伴侣蛋白Skp、DegP、PpiD、YfgM单独和PpiD/YfgM同时敲除后,对双组分蛋白FhaB^*蛋白的分泌基本无影响;而单独敲除分子伴侣蛋白SurA或双敲除Skp/DegP则显著影响了FhaB^*蛋白跨内膜分泌至周质空间的过程。结论革兰阴性菌中可能存在由SurA蛋白介导或由Skp和DegP蛋白共同介导的两个分子伴侣途径,以介导双组分蛋白分泌系统的底物蛋白跨内膜分泌至周质空间。

关键词: 双组分蛋白转运系统, 分子伴侣, 周质空间, 基因敲除, 革兰阴性菌

Abstract: Objective To investigate the function of general chaperones in the secretion of two-partner-secretion (TPS) protein across the inner membrane to the periplasm of Gram-negative bacteria. Methods The general chaperones were knocked-out and the strains were used to prepare spheroplast to see the effects on the secretion of FhaB^*, which is a substrate of the representative model TPS substrates FhaB/FhaC system. Results Single knock-out of chaperones Skp,DegP,PpiD,YfgM and double knock-out of PpiD/YfgM has no or little effect on the secretion of FhaB^* into the periplasm, while single knock-out of SurA or double knock-out of Skp/DegP severely affect the secretion of FhaB^*. Conclusions There may be two molecular chaperone pathways mediated by SurA or by both SKP and DegP in Gram-negative bacteria, which can mediate the substrate protein of the two-partner-secretion system secretes into the peripheral space.

Key words: two-partner-secretion, molecular chaperone, periplasm, gene knock-out, Gram-negative bacteria

中图分类号:

R378.99

张艳娟, 王哲, 蔡锟, 黄丕英, 储引娣, 范恩国. 革兰阴性菌中分子伴侣在双组分蛋白分泌至周质空间过程中的作用[J]. 基础医学与临床, 2020, 40(4): 456-459.

ZHANG Yan-juan, WANG Zhe, CAI Kun, Huang Pi-ying, CHU Yin-di, FAN En-guo. Function of molecular chaperones in the secretion of two-partner-secretion to the periplasm in Gram-negative bacteria[J]. Basic & Clinical Medicine, 2020, 40(4): 456-459.

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