TRPM7干扰载体修复低氧/复氧致大鼠心肌细胞系H9C2损伤

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (2): 209-214.

TRPM7干扰载体修复低氧/复氧致大鼠心肌细胞系H9C2损伤

杨崛圣¹, 樊虎熊³, 胡姗², 高日峰³, 卢飞⁴, 王恒⁵, 唐燕华^1*

1.南昌大学第二附属医院心脏大血管外科, 江西南昌 330031;
2.南昌大学第二附属医院麻醉科, 江西南昌 330031;
3.南昌大学第二附属医院南昌大学研究学院医学部, 江西南昌 330031;
4.南昌大学第二附属医院南昌大学第二附属医院介入室, 江西南昌 330031;
5.平顶山市第二人民医院胸心外科,河南平顶山 467000

收稿日期:2019-05-20 修回日期:2019-06-26 出版日期:2020-02-05 发布日期:2020-02-05
通讯作者: ^*tyh8565@163.com

TRPM7 interference vector repaires injury caused by hypoxia/reoxygenation in cardiomyocyte cell line H9C2

YANG Jue-sheng¹, FAN Hu-xiong³, HU Shan², GAO Ri-feng³, LU Fei⁴, WANG Heng⁵, TANG Yan-hua^1*

1. Department of Cardiovascular Surgery;
2. Department of Anesthesiology, the Second Affiliated Hospital of Nanchang University;
3. Medical Department of Graduate School of Nanchang University;
4. Department of Interventional , the Second Affiliated Hospital of Nanchang University, Nanchang 330031;
5. Department of Cardiothoracic Surgery, Pingdingshan Second People's Hospital, Pingdingshan 467000, China

Received:2019-05-20 Revised:2019-06-26 Online:2020-02-05 Published:2020-02-05
Contact: ^*tyh8565@163.com

摘要/Abstract

摘要： 目的研究瞬时受体电位通道 7(TRPM7)干扰载体对低氧大鼠心肌细胞系(H9C2)修复功能的影响及其机理。方法建立 H9C2心肌细胞低氧/复氧模型,构建 TRPM7 干扰表达载体转染H9C2细胞,用RT-qPCR与Western blot检测H9C2细胞低氧诱导因子(HIF1-α) 和 TRPM7 的表达,流式细胞计量术检测胞内钙离子水平([Ca²⁺]_i)和细胞凋亡,确定 TRPM7 对H9C2心肌细胞的修复作用。结果 H9C2 细胞转染 TRPM7 干扰载体后,细胞的HIF1-α和TRPM7 表达显著下降(P＜0.05),[Ca²⁺]_i 降低及凋亡率减少(P＜0.05)。结论 TRPM7 干扰载体可能通过 PI3K/Akt 通路对低氧 H9C2 心肌细胞有显著的修复作用。

关键词: TRPM7干扰载体, [Ca²⁺]_i, 细胞凋亡

Abstract: Objective To study the repair function and mechanism of TRPM7 interference vector in hypoxic myocardial cell line (H9C2). Methods The hypoxia/reoxygenation model of H9C2 cardiomyocytes was established and the TRPM7 interference expression vector was constructed to transfect H9C2 cells. The expression of HIF1-α and TRPM7 in H9C2 cells was detected by RT-qPCR and Western blot. [Ca²⁺]_i and apoptosis was determined by flow cytometry. Results When H9C2 cells were transfected with TRPM7 interference vector, the expression of HIF1-α TTRPM7, the [Ca²⁺]_i concentration and the apoptosis rate all decreased. Conclusions TRPM7 interference vector has significant repairing effect on hypoxia H9C2 cardiomyocytes, and the mechanism may be mediated PI3K/Akt pathway.

Key words: TRPM7 interference vector, [Ca²⁺]_i, cell apoptosis

中图分类号:

R602

杨崛圣, 樊虎熊, 胡姗, 高日峰, 卢飞, 王恒, 唐燕华. TRPM7干扰载体修复低氧/复氧致大鼠心肌细胞系H9C2损伤[J]. 基础医学与临床, 2020, 40(2): 209-214.

YANG Jue-sheng, FAN Hu-xiong, HU Shan, GAO Ri-feng, LU Fei, WANG Heng, TANG Yan-hua. TRPM7 interference vector repaires injury caused by hypoxia/reoxygenation in cardiomyocyte cell line H9C2[J]. Basic & Clinical Medicine, 2020, 40(2): 209-214.

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