白芍总苷对EAT小鼠Treg/Th17免疫平衡的影响

基础医学与临床 ›› 2019, Vol. 39 ›› Issue (8): 1157-1162.

白芍总苷对EAT小鼠Treg/Th17免疫平衡的影响

童芳,刘辉,董群,王萍

皖南医学院

收稿日期:2018-06-07 修回日期:2018-10-30 出版日期:2019-08-05 发布日期:2019-07-16
通讯作者: 王萍 E-mail:wangping_152@163.com

Effect of total glucosides of paeonia on Treg/Th17 immune balance in EAT mice

Received:2018-06-07 Revised:2018-10-30 Online:2019-08-05 Published:2019-07-16

摘要/Abstract

摘要： 目的建立实验性自身免疫性甲状腺炎(EAT)小鼠模型，观察不同剂量白芍总苷（TGP）治疗组小鼠脾细胞中调节性T细胞（Treg）和Th17细胞比例及其功能的变化。方法将EAT模型小鼠随机分为模型（model）组、高剂量TGP (THD)组、中剂量TGP (TMD)组、低剂量TGP(TLD)组和雷公藤多苷(TII)组5 组；real-time PCR检测脾细胞中Foxp3 mRNA和ROR?t mRNA的表达；流式细胞计量术检测脾细胞中Treg和Th17细胞的比例；ELISA检测血清中TGF-?1和IL-17的水平；化学发光法检测血清中抗甲状腺球蛋白抗体（TGAb）的水平。结果 TGP处理组（包括低、中和高剂量组）Th17细胞比例较模型组明显降低（P＜0.001）；中和高剂量TGP组Foxp3 mRNA表达量较模型组明显增高( P＜0.05)； Treg细胞比例较模型组明显增高(P＜0.01，P＜0.05) ；IL-17浓度较模型组明显降低(P＜0.01，P＜0.05)；TGAb浓度较模型组明显降低(P＜0.05)；中剂量TPG组TGF-?1浓度较模型组显著增高（P＜0.01）。结论 TGP能调节Treg/Th17免疫平衡，有利于EAT疾病的转归, 其中以中和高剂量TGP组疗效显著。

关键词: EAT, TGAb, 白芍总苷, Treg/Th17免疫平衡, 雷公藤多苷

Abstract: Objective An experimental autoimmune thyroiditis (EAT) model in mice was established to evaluate the proportion and function of regulatory T cells (Tregs) and Th17 cells in the splenocytes from the treatment group with different doses of total glucosides of paeony (TGP) . Methods The EAT model mice were randomly divided into 5 groups: model group, TGP high-dose group, TGP medium-dose group,TGP low-dose group and Tripterygium glycosides group. Real-time PCR was performed to detect the expression of Foxp3 mRNA and ROR?t mRNA in the splenocytes of each group. Flow cytometry was performed to detect the percentage of Treg and Th17 cells in the mice splenocytes of each group. Serum levels of TGF-?1 and IL-17 in each group were measured by ELISA. Serum levels of TGAb in each group were detected by chemiluminescence assay.Results Foxp3 mRNA expression in the mice splenocytes in the medium-dose and the high-dose TGP group were significantly higher than that in the model group(P< 0.05). The percentages of Treg cells in the medium-dose and the high-dose TGP group were significantly higher than that in the model group( P<0.01,P<0.05). The percentages of T cells secreting IL-17 in the low-, medium- and high-dose groups of TGP were significantly lower than that in the model group(P<0.001). Serum levels of TGF-?1 in the TGP medium-dose group and the TGP high-dose group were significantly higher than that in the model group( P<0.01).Serum levels of IL-17 in the TGP medium-dose group and the TGP high-dose group were significantly lower than that in the model group (P<0.01,P<0.05). Serum levels of TGAb in the TGP medium-dose group and the TGP high-dose group were significantly lower than that in the model group (P<0.05). Conclusions TGP can regulate the Treg/Th17 immune balance, which can be beneficial to the prognosis of EAT disease. The TGP medium-dose group and the TGP high-dose group had obvious curative effect.

Key words: Experimental Autoimmune Thyroiditis, Thyroglobulin antibody, Total Glucosides of Paeony, Treg/Th17 immune balance, Tripterygium glycosides

童芳刘辉董群王萍. 白芍总苷对EAT小鼠Treg/Th17免疫平衡的影响[J]. 基础医学与临床, 2019, 39(8): 1157-1162.