关键词: 人脂肪间充质干细胞, 炎性细胞因子, 免疫调控, 银屑病

Abstract: Objective To study the effects of tectoridin and aucubin on the immune regulation of human adipose mesenchymal stem cells (hAMSCs) induced by lipopolysaccharide (LPS).Methods CCK-8 cell proliferation test and cell cycle test were used to screen the appropriate concentration of drugs; LPS concentration was screened to establish an inflammatory response model; ELISA and RT-q PCR were used to detect the effects of both on the secretion of pro-inflammatory and anti-inflammatory factors by hAMSCs after LPS induction. Results 50μg/mL tectoridin and 100μg/mL aucubin could significantly promote the proliferation of normal hAMSCs (P < 0.05 ，P＜0.01). Both of them promoted the proliferation of hAMSCs induced by LPS (P<0.001), and inhibited the secretion of IL-6，TNF-α，IFN-γ (P＜0.05 ，P＜0.01) .Conclusions Tectoridin and aucubin could significantly inhibit LPS-induced hAMSCs inflammatory response.

Key words: human adipose mesenchymal stem cells, inflammatory cytokines, immune regulation, psoriasis