基础医学与临床 ›› 2019, Vol. 39 ›› Issue (7): 1066-1070.
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周芳颖,刘玉琴
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摘要： 饲养层细胞法被广泛应用于干细胞及其它哺乳动物细胞的体外培养，通过分泌生长因子和物理支持可以一定程度模拟细胞增殖的体内环境。根据培养目的的不同，可以有选择性的挑选饲养层细胞种类、处理方法，或通过基因编辑对饲养层细胞进行改良，从而达到最佳的培养效果。
关键词: 饲养层细胞, 基因修饰, 干细胞及诱导多能干细胞
Abstract: Feeder cells have been widely used as an assistant method for the in vitro culture of mammal cells.It can simulate the condition of in vivo culture to a certain extent by the secretion of growth factors and the physical support. According to different culture purposes,we may choose specific feeder cells and dealing methods or modify feeder cells through gene editing to obtain the best culture results.
Key words: Feeder cells, Gene editing, Stem cell and iPS
周芳颖 刘玉琴. 饲养层细胞作用机制研究进展[J]. 基础医学与临床, 2019, 39(7): 1066-1070.
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https://journal11.magtechjournal.com/Jwk_jcyxylc/CN/Y2019/V39/I7/1066
