基础医学与临床 ›› 2019, Vol. 39 ›› Issue (7): 1007-1012.

• 研究论文 • 上一篇    下一篇

人工与野生蛹虫草虫草素和多糖含量及其水提物对食管癌细胞抗癌活性的影响

周瑞琴,张诚,罗骏,叶琳   

  1. 重庆医科大学附属第一医院
  • 收稿日期:2018-04-23 修回日期:2018-10-11 出版日期:2019-07-05 发布日期:2019-07-02
  • 通讯作者: 周瑞琴 E-mail:18983149940@163.com

Effects of the content of cordycepin, polysaccharides and water extract of cultivated and wild cordyceps militaris on anti-esophageal cancer cells activity

  • Received:2018-04-23 Revised:2018-10-11 Online:2019-07-05 Published:2019-07-02

摘要: 目的 比较人工与野生蛹虫草素和多糖含量及其水提物对食管癌细胞的抗癌活性,并建立蛹虫草质量评价体系。方法 用HPLC技术检测水提物中虫草素的含量,色谱条件为:Agilent ZORBAX SB-C18液相色谱柱(250 mm×4.6 mm×5 μm ),流动相为甲醇/水(15/85,v/v ),流速为1 mL/min,柱温25 ℃检测波长260 nm,进样量1 μL;用苯酚-硫酸法检测虫草多糖的含量;MTS法检测细胞增殖,用Annexin V-PI双染流式细胞计量术检测细胞凋亡。结果 人工虫草和野生虫草的虫草素含量分别为400 μg/g和29 μg/g;虫草多糖的含量分别为4.26%和3.12%;人工虫草水提物抑制肿瘤细胞的增殖和诱导凋亡的作用强于野生虫草(P<0.05)。 结论 人工培育的蛹虫草在虫草素和多糖含量及其水提物抗癌活性方面均优于野生蛹虫草,说明人工培育的蛹虫草是野生虫草良好的替代品。

关键词: 人工蛹虫草, 野生蛹虫草, 食管癌细胞, 虫草素, 虫草多糖, 抗癌活性

Abstract: Objectiv By comparison of the content of cordycepin, polysaccharides and he anti-esophageal cancer cells activity of water extract of cultivated Cordyceps militaris and wild Cordyceps militaris, a preliminary study on the quality identification of Cordyceps militaris was carried out to provide certain reference value for the establishment of the quality evaluation system of Cordyceps militaris. Methods Using HPLC technology to detect the content of cordycepin in Cultivated Cordyceps militaris and Wild Cordyceps militaris. HPLC conditions—column: Zorbax SB-C18, 5μm, 250 mm×4.6 mm, Agi-lent, USA; the mobile phases: methanol (M,15% ) and water (W,85% ); flowing rate: 1.0 ml/min;temperature: 25℃; detection wave length: 260 nm; injection: 1μL. Phenol-sulfuric acid method was used to detect the content of polysaccharides. By MTS assay we detected the condition of cell proliferation treated with different concentrations of WECM. Annexin V/PI flow cytometry was used to determine the apoptosis of EC109 cells. Result HPLC results showed that the content of cordycepin in cultivated Cordyceps militaris and wild Cordyceps militaris ware 400.34 μg/g and 28.63 μg/g; the content of polysaccharides in cultivated Cordyceps militaris and wild Cordyceps militaris ware 4.256% and 3.115%,assaying by optimized phenol-sulfuric acid method; The results of MTS and flow cytometry indicated that the anticancer activity of the cultivated Cordyceps militaris was obviously more than wild Cordyceps militaris. Conclusion cultivated Cordyceps militaris is better than wild Cordyceps militaris in the the content of cordycepin, polysaccharides and the anticancer activity of water extract of Cordyceps militaris, it is indicated that Cultivated Cordyceps militaris is a good substitute for wild Cordyceps militaris.

Key words: Cultivated Cordyceps militaris, Wild Cordyceps militaris, Esophageal cancer cells, cordycepin, polysaccharides, anticancer activity