鸟氨酸脱羧酶抗酶-1过表达促进非小细胞肺癌细胞系A549凋亡

基础医学与临床 ›› 2019, Vol. 39 ›› Issue (3): 332-336.

鸟氨酸脱羧酶抗酶-1过表达促进非小细胞肺癌细胞系A549凋亡

欧洁¹,王顺超¹,孙丽丹¹,曹春雨²,吴红艳¹

1. 三峡大学
2. 三峡大学肿瘤微环境与免疫治疗湖北省重点实验室

收稿日期:2018-05-07 修回日期:2018-07-22 出版日期:2019-03-05 发布日期:2019-03-05
通讯作者: 吴红艳 E-mail:wuhongyan@ctgu.edu.cn
基金资助:
国家自然科学基金

Over-expression of ornithine decarboxylase antienzyme-1 promotes the apoptosis of non-small cell lung cancer cell line A549

Received:2018-05-07 Revised:2018-07-22 Online:2019-03-05 Published:2019-03-05

摘要/Abstract

摘要： 目的探讨上调鸟氨酸脱羧酶（ODC）抗酶-1(AZ-1)对非小细胞肺癌A549多胺代谢酶表达和细胞凋亡的影响。方法制备靶向提高人源性AZ-1表达的pcDNA3.1-HOAZ1质粒，用其与空载质粒pcDNA3.1（-）分别转染A549细胞系，并设立未经任何处理的空白对照组，48 h后分别收集总RNA和蛋白质，通过反转录聚合酶链反应和蛋白质印记分析分别检测AZ-1、ODC和鸟氨酸脱羧酶抗酶抑制剂-1（AZIN-1）的表达；同样的处理条件下应用流式细胞计量术检测细胞凋亡。结果在转染pcDNA3.1-HOAZ1质粒后，ODC表达被明显抑制，AZIN-1的表达调节不明显；过表达AZ-1引起细胞凋亡增加(P<0.05)。结论过表达AZ-1能抑制ODC表达和促进A549细胞凋亡。

关键词: 鸟氨酸脱羧酶抗酶-1, 鸟氨酸脱羧酶, 多胺, 非小细胞肺癌细胞

Abstract: Objective To evaluate whether up-regulating ornithine decarboxylase （ODC）antizyme-1 (AZ-1)can regulate the expression of polyamine metabolic enzymes and proliferation of non-small cell lung cancer A549 or not. Methods A549 cell line was transfected with pcDNA3.1-HOAZ1 and pcDNA3.1 respectively and a blank control group without any treatment was established, total RNA and protein were collected after 48 hours. The expression of AZ-1, ODC and ornithine decarboxylase anti-enzyme inhibitor-1（AZIN-1）were examined by RT-PCR and Western blot respectively. Flow cytometry was used to detect apoptosis in the same treatment conditions. Results After transfected with pcDNA3.1-HOAZ1 plasmid, the expression of ODC was significantly inhibited, and the expression of AZIN-1 was not significantly regulated. Up-regulating AZ-1 caused apoptosis (P<0.05).

Key words: ornithine decarboxylase antizyme-1, ornithine decarboxylase, polyamines, non-small cell lung cancer

欧洁王顺超孙丽丹曹春雨吴红艳. 鸟氨酸脱羧酶抗酶-1过表达促进非小细胞肺癌细胞系A549凋亡[J]. 基础医学与临床, 2019, 39(3): 332-336.