大鼠中脑导水管周围灰质腹外侧区GABAAα1表达上调与甲醛致急性疼痛有关

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (3): 381-384.

大鼠中脑导水管周围灰质腹外侧区GABAAα1表达上调与甲醛致急性疼痛有关

孙万秋¹,王桂芝²,娄超³,张春燕⁴,冀玉萍¹,李军¹,于剑锋¹

1. 潍坊医学院麻醉学系
2. 山东省潍坊医学院
3. 泰山医学院附属医院
4. 潍坊医学院附属医院

收稿日期:2017-11-20 修回日期:2017-12-19 出版日期:2018-03-05 发布日期:2018-02-27
通讯作者: 王桂芝 E-mail:wgz@wfmc.edu.cn
基金资助:
潍坊医学院科技创新基金

Up-regulation of GABAAα1 in ventrolateral periaqueductal gray in rats is associated with formalin-induced acute pain

Received:2017-11-20 Revised:2017-12-19 Online:2018-03-05 Published:2018-02-27
Contact: Gui-Zhi WANG E-mail:wgz@wfmc.edu.cn

摘要/Abstract

摘要： 目的探讨甲醛致急性疼痛大鼠中脑导水管周围灰质腹外侧区（vLPAG）γ-氨基丁酸A受体α1亚基（GABAAα1）的表达变化。方法将大鼠随机分为对照组（C组，n=12）和甲醛致痛组（F组，n=12）：于右侧后足底注射0.9%氯化钠溶液和2%甲醛各50 μL。记录大鼠建模后1 h内每5 min的缩足舔爪时间，分别统计两时相缩足舔爪时间之和，作为大鼠疼痛学评分；记录大鼠建模后1 h内每10 min各时间点的机械痛阈；1 h后处死大鼠，用蛋白免疫印迹法检测各组vLPAG处GABAAα1相对表达量。结果 F组大鼠出现了明显的缩足舔爪的急性疼痛表现，且呈两相性，疼痛学评分均高于C组（P<0.05）；机械痛阈各时点均低于C组（P<0.05）；F组GABAAα1蛋白表达含量较C组明显升高（P<0.05）。结论中脑导水管周围灰质腹外侧区GABAAα1表达上调与大鼠急性疼痛痛阈降低有关。

关键词: 关键词：急性疼痛, 中脑导水管周围灰质腹外侧区, GABAA受体α1亚基

Abstract: Objective To investigate the expression of γ-aminobutyric acid A receptor α1 subunit ( GABAAα1 ) in the ventrolateral periaqueductal gray ( vLPAG ) in rats with formalin-induced acute pain. Methods The rats were randomly divided into two groups: control group ( group C ) and formalin-induced pain group (group F)，12 rats in each group: 0.9% sodium chloride solution or 2% formaldehyde 50 μL was injected into the ventral surface of right hind paw respectively. The pain scores were recorded for every 5 minutes and the mechanical pain threshold were recorded for every 10 minutes until 1 h. The expression levels of GABAAα1 in vLPAG were determined by Western blotting analysis in each group. Results The rats in formalin group showed significant nociceptive behaviors immediately, such as paw withdrawal and/or paw licking. Results demonstrated that the rats exhibited a biphasic response to pain. The pain behavior scores in group F were significantly higher than that in group C ( P <0.05 ), and the mechanical pain threshold in group F was decreased after injection compared with group C ( P <0.05 ). The expression of GABAAα1 protein in group F was significantly higher than that in group C ( P <0.05 ). Conclusion The up-regulation of GABAAα1 expression in ventrolateral periaqueductal gray is associated with the decrease of pain threshold in rats with acute pain.

Key words: Keywords：Acute pain, Ventrolateral periaqueductal gray, vLPAG, GABAAα1

中图分类号:

R338.2
R-332

孙万秋王桂芝娄超张春燕冀玉萍李军于剑锋. 大鼠中脑导水管周围灰质腹外侧区GABAAα1表达上调与甲醛致急性疼痛有关[J]. 基础医学与临床, 2018, 38(3): 381-384.