阿托伐他汀对糖尿病大鼠肾组织BMP-7和SnoN表达的影响

基础医学与临床 ›› 2018, Vol. 38 ›› Issue (10): 1373-1377.

• 研究论文 • 下一篇

阿托伐他汀对糖尿病大鼠肾组织BMP-7和SnoN表达的影响

彭伟¹,张小欢²,刘玲伶³,王圆圆²,刘丽荣⁴,石明隽²,郭兵²

1. 贵州医科大学
2. 贵州医科大学病理生理学教研室
3. 贵州省贵州医科大学
4. 贵阳医学院

收稿日期:2017-10-09 修回日期:2017-12-24 出版日期:2018-10-05 发布日期:2018-09-28
通讯作者: 刘丽荣 E-mail:896831978@qq.com
基金资助:
国家自然科学基金;教育部博导项目;贵州省科技厅联合基金

Atorvastatin promotes the expression of bone morphology protein-7 and SnoN in the kidney of diabetic rats

Received:2017-10-09 Revised:2017-12-24 Online:2018-10-05 Published:2018-09-28

摘要/Abstract

摘要： 目的观察阿托伐他汀（atorvastatin，AT）治疗对糖尿病大鼠肾组织骨形态发生蛋白-7（BMP-7）和核转录共抑制因子SnoN表达的影响。方法将大鼠分为对照组（NC组）、糖尿病组（DM组）（用链脲佐菌素（STZ）复制糖尿病大鼠模型）和糖尿病+阿托伐他汀灌胃治疗组（AT组），每组n=8。16周处死大鼠，检测生化指标，观察肾组织病理学改变，免疫组化检测肾组织胶原蛋白I（ColI）和胶原蛋白III（ColIII）的表达，Western blot检测肾组织BMP-7、SnoN、E-钙联蛋白（E-cadherin）、骨架蛋白（Desmin）和胶原蛋白IV（ColIV）的表达。结果与对照组相比，DM组大鼠的血糖、三酰甘油、肾脏指数、尿微量白蛋白/尿肌酐（MA/CR）均增多（P<0.05）；AT组大鼠上述指标回降（P<0.05）。DM组出现了肾小球萎缩，肾小管炎性浸润等典型纤维化病变，AT组病变有所减轻。DM组CoI和CoIII增多，AT组回降。与对照组相比，DM组BMP-7、SnoN、E-cadherin表达显著减少，而Desmin和ColIV表达增多，AT组BMP-7、SnoN、E-cadherin表达回升（P<0.05），而Desmin和ColIV表达回降（P<0.05）。结论阿托伐他汀可以通过上调BMP-7 和SnoN的蛋白表达来治疗或延缓糖尿病肾病。

关键词: 关键词：糖尿病肾病, 骨形态发生蛋白-7, SnoN, EMT

Abstract: Objective To observe the effects of Atorvastatin on the expression of Bone morphology protein-7 and SnoN in the kidney of diabetic rats. Methods The rats were divided into normal control（NC） group，diabetic (DM) group and atorvastatin lavage treatment (AT) group randomly.After 16 weeks,the rats were sacrificed to measure biochemical parameters ,and to observe the changes of pathomorphology.Meanwhile,immunohistochemistry and Western blot were employed to examine protein expression of collagen-I（Col-I）,collagen-III（Col-III）,BMP-7,SnoN,E-cadherin,Desmin and collagen-IV（Col-IV）in the renal tissue.Results Compared with NC group, the levels of blood glucose(BG), triglyceride(TG), kidney index and the urine trace albumin/uric creatinine (MA/CR) were increased remarkably in DM group（P<0.05）. In AT group ,the levels of BG, TG, MA/CR were reduced（P<0.05）. The glomerular atrophy was observed in the DM group compared with the NC group, and the alleviated renal fibrosis was observed in AT group. Compare with NC group, the expression of BMP-7, SnoN, E-cadherin were significantly decreased in DM group, the expression of Desmin, ColI, ColIII and ColIV were a significantly increased in DM group（P<0.05）. In vivo，compare with DM group, Atorvastatin increase the expression of BMP-7, SnoN, E-cadherin the expression of Desmin，ColI,，ColIII and ColIV were a significantly decreased in AT group（P<0.05）. Conclusion Atorvastatin promotes the expression of BMP-7 and SnoN ,therefore protect the kidney of diabetic rats.

Key words: KEY WORDS：diabetic nephropathy, BMP-7, SnoN, EMT

彭伟张小欢刘玲伶王圆圆刘丽荣石明隽郭兵. 阿托伐他汀对糖尿病大鼠肾组织BMP-7和SnoN表达的影响[J]. 基础医学与临床, 2018, 38(10): 1373-1377.

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阿托伐他汀对糖尿病大鼠肾组织BMP-7和SnoN表达的影响

Atorvastatin promotes the expression of bone morphology protein-7 and SnoN in the kidney of diabetic rats

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