帕金森病大鼠模型十二指肠黏膜紧密连接蛋白表达下调

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (10): 1359-1362.

帕金森病大鼠模型十二指肠黏膜紧密连接蛋白表达下调

张冬妮¹,王忆安²,李广文²,闫力铭²,冯晓燕¹,朱进霞³

1. 首都医科大学
2. 首都医科大学基础医学院
3. 首都医科大学基础医学院生理学系

收稿日期:2017-04-21 修回日期:2017-06-29 出版日期:2017-10-05 发布日期:2017-09-25
通讯作者: 冯晓燕 E-mail:fengxy1126@163.com
基金资助:
国家自然科学基金

Down-regulated expression of duodenal tight junction proteins in model rats with Parkinson's disease

Received:2017-04-21 Revised:2017-06-29 Online:2017-10-05 Published:2017-09-25

摘要/Abstract

摘要： 目的研究紧密连接蛋白在6-羟多巴（6-OHDA）制备的帕金森病（PD）大鼠模型十二指肠黏膜的表达变化。方法用6-OHDA损毁双侧中枢黑质多巴胺能神经元建立大鼠模型。用免疫荧光组织化学和蛋白免疫印迹检测紧密连接蛋白claudin-1、occludin和ZO-1肠黏膜的定位和表达。结果紧密连接蛋白claudin-1、occludin和ZO-1在PD大鼠模型十二指肠黏膜上均有表达，但仅ZO-1（P<0.001）和occludin（P<0.01）表达明显下调，而claudin-1无显著变化。结论 PD大鼠模型十二指肠黏膜紧密连接蛋白ZO-1、occludin的表达显著下调，可能与帕金森病十二指肠溃疡的发生发展相关。

关键词: 关键词 6-羟多巴大鼠模型, 十二指肠, Claudin-14, Occludin, ZO-1

Abstract: Objective To investigate the alterations of tight junction proteins in the duodenal mucosa of 6-hydroxydopamine (6-OHDA)-induced Parkinson’s disease (PD) models. Methods Rats were subjected with 6-OHDA in bilateral substantia nigra (SN) to make the PD models. The location and quantitative detecting techniques including immunohisto- chemistry and western blotting were used to determine the expressions and alterations of tight junction proteins (claudin-1, occludin, ZO-1). Results Claudin-1, occludin and ZO-1 were highly expressed in the duodenal mucosa of PD models, where the expressions of occluding (P<0.001) and ZO-1 (P<0.001) were much lower, while the expression of claudin-1 had no alteration. Conclusions The down-regulated expressions of occludin and ZO-1 were detected in duodenal mucosa of PD models, which might be related with duodenal ulcer in PD.

Key words: Key words: 6-OHDA models, duodenum, claudin-1, occluding, ZO-1

中图分类号:

R 592

张冬妮王忆安李广文闫力铭冯晓燕朱进霞. 帕金森病大鼠模型十二指肠黏膜紧密连接蛋白表达下调[J]. 基础医学与临床, 2017, 37(10): 1359-1362.