羟基红花黄色素A抑制H22小鼠肝癌移植瘤血管生成及减少MMP-3的表达

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (1): 71-75.

羟基红花黄色素A抑制H22小鼠肝癌移植瘤血管生成及减少MMP-3的表达

张静宇¹,杨芳芳²,李京敏¹,刘春晓³,白咸勇¹

1. 滨州医学院
2. 滨州医学院附属医院
3. 莒县中医院

收稿日期:2016-08-29 修回日期:2016-11-01 出版日期:2017-01-05 发布日期:2016-12-30
通讯作者: 白咸勇 E-mail:xybai1963@126.com
基金资助:
羟基红花黄色素A对肝细胞癌炎症和血管生成的调控作用及机制研究;羟基红花黄色素A对肝细胞癌血管生成及p38MAPK信号通路的作用研究

HSYA inhibits angiogenesis of H22 transplanted tumor tissue and down-regulation of MMP-3 expression in mice

Received:2016-08-29 Revised:2016-11-01 Online:2017-01-05 Published:2016-12-30

摘要/Abstract

摘要： 目的研究红花组分羟基红花黄色素A（HSYA）对H22小鼠肝癌移植瘤组织新血管生成的抑制作用及对基质金属蛋白酶-3（MMP-3）的影响。方法建立H22小鼠肝癌移植瘤模型，第2天将小鼠随机分为对照组、索拉非尼组及HSYA组（1.125和2.25mg/kg），HE观察肝癌移植瘤组织病理；用免疫组织化学法检测肿瘤组织中CD34的表达，计数微血管密度（MVD）；免疫组化和Western blot分别检测瘤组织中MMP-3的表达。结果与对照组相比，HSYA组（1.125和2.25mg/kg）的MVD显著降低（p<0.01），MMP-3蛋白在移植瘤组织中的表达明显减少（p<0.01），以HSYA组（2.25mg/kg）效果最为显著,但不及索拉非尼组。结论 HSYA在一定浓度范围内抑制H22小鼠肝癌移植瘤血管生成，其作用可能与降低基质金属蛋白酶-3的蛋白表达有关。

关键词: 羟基红花黄色素A，基质金属蛋白酶-3，肝细胞癌，肿瘤血管生成

Abstract: Abstract: Aim: To investigate the effects of HydroxySafflor yellow A(HSYA) on the protein expression of MMP-3 in H22 tumor-bearing mice.Methods:After establishing the hepatoma model for 24 h, the mice were randomly divided into 4 groups. The vehicle-treated group received 0.9% normal saline via intraperitoneal injection. The positive control group was treated with sorafenib by intragastric administration. And the dose HSYA group received intraperitoneal injection at different dosages (1.125,2.25mg/kg).Immunohistochemical staining and western blotting were applied to measure the expression of angiogenesis related factor（MMP-3）and we also detected the microvessel density with CD34.Results:Compared with saline group, the expression of MMP-3 in HSYA group was significant reduced. Especially the dose of 2.25mg/kg HSYA group, but not better than the sorafenib-treated group.Conclusion:HSYA could inhibit angiogenesis of tumor tissue in a certain concentration range and the anti angiogenesis effect of HSYA may be related to the decrease of the protein expression of matrix metalloproteinase -3.

Key words: Hydroxysafflor yellow A, MMP-3, Hepatocellular carcinoma, Tumor angiogenesis

张静宇杨芳芳李京敏刘春晓白咸勇. 羟基红花黄色素A抑制H22小鼠肝癌移植瘤血管生成及减少MMP-3的表达[J]. 基础医学与临床, 2017, 37(1): 71-75.