miR-551b-3p在胃癌组织中低表达并抑制胃癌细胞系HGC-27增殖、迁移和侵袭

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (5): 610-614.

miR-551b-3p在胃癌组织中低表达并抑制胃癌细胞系HGC-27增殖、迁移和侵袭

温馨,翟頔,王芳,余佳

中国医学科学院基础医学研究所北京协和医学院基础学院

收稿日期:2016-02-22 修回日期:2016-03-17 出版日期:2016-05-05 发布日期:2016-04-26
通讯作者: 余佳 E-mail:j-yu@ibms.pumc.edu.cn;jyu403@gmail.com
基金资助:
国家自然科学基金重大研究计划;国家自然科学基金面上项目

miR-551b-3p decreases in gastric cancer tissue and inhibits the proliferation, migration and invasion of gastric cancer cell line HGC-27

,Di ZHAI,Fang WANG,Jia YU

Received:2016-02-22 Revised:2016-03-17 Online:2016-05-05 Published:2016-04-26
Contact: Jia YU E-mail:j-yu@ibms.pumc.edu.cn;jyu403@gmail.com

摘要/Abstract

摘要： 目的探讨miR-551b-3p在胃癌中的表达变化情况，及其对胃癌细胞功能的影响。方法用real-time PCR的方法检测60例胃癌组织及其对应的癌旁组织中miR-551b-3p的表达量，使用miR-551b-3p模拟物（miR-551b-3p mimic）转染胃癌细胞系HGC-27，CCK-8法检测细胞增殖；划痕愈合实验检测细胞迁移；Transwell法检测细胞侵袭。结果 1）miR-551b-3p在胃癌癌症组织中的表达明显低于癌旁组织（P<0.05）。2）过表达miR-551b-3p mimic可以明显减弱胃癌细胞系HGC-27的增殖、迁移和侵袭能力。结论 miR-551b-3p在胃癌组织中低表达，并且抑制胃癌细胞系HGC-27的增殖、迁移和侵袭，可能与胃癌发生发展密切相关。

关键词: miR-551b-3p, 胃癌, 增殖、迁移和侵袭

Abstract: Objective To investigate the expression and the expression change of miR-551b-3p in gastric cancer and its effect on the function of gastric cancer cells. Methods miR-551b-3p was detected by RT-PCR in 60 cases of gastric cancer samples and their paired adjacent normal control tissues. HGC-27 cell line was infected by miR-551b-3p mimic, then CCK-8 assay was used to determine the influence of miR-551b-3p on HGC-27 cell proliferation; Wound-healing assay was used to determine cell migration; Transwell assay was used to determine cell invasion. Results 1) The expression of miR-551b-3p in gastric tumor tissues was significantly lower than that of the adjacent tissues (P<0.05). 2) HGC-27 cell proliferation, migration and invasion were significantly decreased while the cell infected by mimic-miR-551b-3p. Conclusions miR-551b-3p expresses lower in gastric cancer tissues and is able to inhibit gastric cancer line HGC-27 proliferation, migration and invasion. miR-551b-3p is closely associated with the development of gastric cancer.

Key words: miR-551b-3p, gastric cancer, proliferation, migration and invasion

温馨翟頔王芳余佳. miR-551b-3p在胃癌组织中低表达并抑制胃癌细胞系HGC-27增殖、迁移和侵袭[J]. 基础医学与临床, 2016, 36(5): 610-614.

Di ZHAI Fang WANG Jia YU. miR-551b-3p decreases in gastric cancer tissue and inhibits the proliferation, migration and invasion of gastric cancer cell line HGC-27[J]. Basic & Clinical Medicine, 2016, 36(5): 610-614.

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