过表达CXCR4/CXCR7影响人脐带间充质干细胞迁移和增殖

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (10): 1341-1347.

过表达CXCR4/CXCR7影响人脐带间充质干细胞迁移和增殖

李娅莎¹,杨珂¹,赵丽²,谭彬¹,龚梦嘉³,董世访¹,毕杨⁴

1. 重庆医科大学附属儿童医院
2. 重庆市重庆医科大学附属儿童医院儿科研究所，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室
3. 重庆医科大学
4. 重庆医科大学附属儿童医院儿研所干细胞实验室//儿童发育疾病研究教育部重点实验室//重庆市干细胞治疗工程技术研究中心

收稿日期:2015-12-03 修回日期:2016-01-20 出版日期:2016-10-05 发布日期:2016-09-27
通讯作者: 毕杨 E-mail:yangbi1981@cqmu.edu.cn
基金资助:
携带双自杀基因的可回复永生化肝细胞株优化及应用

Overexpression of CXCR4 /CXCR7 affects migration and proliferation of human umbilical cord mesenchymal stem cells

Received:2015-12-03 Revised:2016-01-20 Online:2016-10-05 Published:2016-09-27
Contact: Yang Bi E-mail:yangbi1981@cqmu.edu.cn

摘要/Abstract

摘要： 目的：探讨SDF-1/CXCR4及SDF-1/CXCR7对人脐带间充质干细胞（hUC-MSCs）迁移和增殖活性的影响。方法：用Ad-EASY腺病毒质粒系统分别构建表达CXCR4和CXCR7的重组腺病毒，感染hUC-MSCs后用FCM分别检测细胞膜的CXCR4和CXCR7受体的表达，Transwell法检测细胞迁移，MTT检测hUC-MSCs增殖活性，以及在H2O2诱导细胞毒性作用对细胞存活的影响。结果：成功构建表达人源的CXCR4和CXCR7的重组腺病毒，感染后hUC-MSCs 细胞膜上的CXCR4/CXCR7受体阳性表达率分别达到93.7%和78.5%（P＜0.01），高表达CXCR4和 CXCR7均显著增强SDF-1诱导的hUC-MSCs细胞迁移，其中CXCR7效应稍弱；高表达CXCR7而不是CXCR4显著提高SDF-1诱导的hUC-MSCs增殖活性和氧化应激状态下的细胞存活率（P＜0.05）。结论：CXCR4/CXCR7均参与介导了SDF-1诱导的hUC-MSCs细胞迁移作用，同时CXCR7还介导了SDF-1诱导的hUC-MSCs增殖和H2O2处理损伤的保护。

关键词: CXCR4/CXCR7, 人脐带间充质干细胞, 细胞迁移, 细胞增殖

Abstract: Objective: To investigate the effect of SDF-1 / CXCR4 and SDF-1/ CXCR7 on the migration and proliferation of human umbilical cord mesenchymal stem cell. Methods: Using Ad-EASY adenoviral vector systems to construct recombinant adenovirus with overexpression of CXCR4 and CXCR7,hUC-MSCs was infected with Ad-CXCR4 or Ad-CXCR7 respectively, FCM was used to detect the expression of CXCR4 and CXCR7 as membrane receptors, Transwell assay was carried out to evaluate the SDF-1 induced migration ability of hUC-MSCs with CXCR4 or CXCR7 overexpression.MTT assay was performed to detect the cell proliferation activity for overexpression CXCR4 or CXCR7 of hUC-MSCs, as well as the cell survival after treated with H2O2. Results:The constructed adenovirus CXCR4 and CXCR7 successfully infected UC-MSCs, and the positive cell rate with membrane expression of CXCR4 or CXCR7 were 93.7% and 78.5%, respectively. Overexpression of CXCR4 and CXCR7 significantly enhanced SDF-1 induced cell migration of hUC-MSCs,which CXCR7 showed weaker effect, then CXCR7 but not CXCR4 was responsible for the SDF-1 induced cell viability and proliferation of hUC-MSCs. Conclusion: Both CXCR4 and CXCR7 mediated the SDF-1 induced migration of hUC-MSCs, in addition CXCR7 mediated the SDF-1 induced hUC-MSCs proliferation and cell viability under anti-oxidative stress.

Key words: CXCR4/CXCR7, Human umbilical cord mesenchymal stem cell, Cell migration, Cell proliferation

中图分类号:

Q291

李娅莎杨珂赵丽谭彬龚梦嘉董世访毕杨. 过表达CXCR4/CXCR7影响人脐带间充质干细胞迁移和增殖[J]. 基础医学与临床, 2016, 36(10): 1341-1347.

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