关键词: 长链非编码RNA, PVT1, 胰腺癌, 上皮间质转化

Abstract: Objective 　To study the effects of down-regulated long non-coding RNA PVT1 expression on proliferation, apoptosis, migration and invasion of pancreatic cancer cells. Methods PVT1 interference sequences siPVT1-1 and siPVT1-2 were transfected into pancreatic cancer cell line BxPC-3 in vitro, and the siPVT1-NC was taken as negative control. The ability of proliferation and apoptosis of BxPC-3 were detected by CCK-8 assay and Flow cytometry, respectively. Wound healing assay and Transwell assay were study the ability of migration and invasion. The mRNA expression of E-cadherin, N-cadherin, β-catenin, vimentin and PVT1 were detected by qRT-PCR. Apoptosis related protein (Bax, Bcl-2, Caspase 3) and epithelial-mesenchymal transition related protein (E-cadherin, N-cadherin, β-catenin, vimentin) were detected by Western blot. Result Down-regulated expression of pancreatic cancer cell line BxPC-3 reduced the ability of proliferation and promoted cell apoptosis (P<0.05). The number of migrated and invased cells were reduced significantly when down-regulated the expression of PVT1 (P<0.05). After transfected with siPVT1-1 and siPVT1-2, the molecular and protein level of E-cadherin were increased, while N-cadherin, β-catenin and vimentin were reduced (P<0.05). The protein level of Bax and Caspase 3 were increased, Bcl-2 was reduced (P<0.05). Conclusion Down-regulated expression of long non-coding RNA PVT1 can reduce cell proliferation and promote cell apoptosis, meanwhile, the ability of migration and invasion are also inhibited. Down-regulated expression of PVT1 can reverse epithelial-mesenchymal transition of pancreatic cancer cells.

Key words: long non-cading RNA, PVT1, pancreatic cancer, epithelial-mesenchymal transition