基础医学与临床 ›› 2015, Vol. 35 ›› Issue (6): 729-733.

• 研究论文 • 上一篇    下一篇

Fcγ和Fcε受体在小鼠三叉神经节的表达

刘帆1,李春艳1,徐鲁斌2,陈纳泽2,周末1,姜浩武1,马超3   

  1. 1. 中国医学科学院基础医学研究所,北京协和医学院基础学院
    2. 北京协和医学院
    3. 中国医学科学院基础医学研究所
  • 收稿日期:2015-03-13 修回日期:2015-04-15 出版日期:2015-06-05 发布日期:2015-05-27
  • 通讯作者: 马超 E-mail:machao@ibms.pumc.edu.cn
  • 基金资助:
    痛觉神经元 Fc-gamma-I 型受体在慢性痛发生中的作用

Expression of Fcγ and Fcε receptor on mouse trigeminal ganglions

  • Received:2015-03-13 Revised:2015-04-15 Online:2015-06-05 Published:2015-05-27

摘要: 目的 检测正常小鼠三叉神经节(TG)是否表达免疫球蛋白G(IgG)和免疫球蛋白E(IgE)Fc段受体Fcγ 受体和 Fcε 受体,及其在过敏小鼠TG中的变化。方法 通过腹腔注射OVA和铝剂,建立小鼠过敏性结膜炎(ACJ)模型。ELISA检测血清总IgE。用Western blot 和免疫荧光检测Fcγ受体和Fcε受体的表达。 结果 小鼠TG表达Fcγ 受体和 Fcε 受体。其中IgG激活型高亲和力受体FcγRI和抑制型低亲和力受体FcγRII只表达在小鼠TG神经元上,而IgG激活型低亲和力受体FcγRIII表达在小鼠TG中的卫星胶质细胞上。IgE激活型高亲和力受体FcεRI表达在小鼠TG神经元上,而IgE低亲和力受体FcεRII同时表达在小鼠TG神经元和卫星胶质细胞上。与正常小鼠比较,ACJ小鼠的血清总IgE水平升高,TG 的FcεRI和FcγRII表达增加(p < 0.05)。而ACJ小鼠的FcεRII和FcγRI表达下降(p < 0.05)。FcγRIII在正常和ACJ小鼠TG中的表达无显著差别。 结论 小鼠TG表达的Fcγ和ε 受体可能参与ACJ及其他过敏性疾病的发生和发展。

关键词: Fcγ 受体, Fcε 受体, 免疫球蛋白G, 免疫球蛋白E, 过敏

Abstract: Objective To investigate the expression of Fcγ and ε receptor on mouse trigeminal ganglions and the expression change f neuronal Fcγ and ε receptor in allergy. Methods Allergy model in mouse was induced by intraperitoneal injection of OVA and Alum. ELISA was applied to identify the level of the serum total IgE in the na?ve and allergic mouse. Western blotting and immunofluorescence were employed to detect the expression and the change of Fcγ and ε receptor on na?ve and allergic mouse trigeminal ganglions. Results Fcγ and ε receptor were expressed on mouse trigeminal ganglions. The FcγRI, the IgG high-affinity activating receptor, and FcγRII that is the low-affinity inhibitory receptor FcγRII were only expressed on trigeminal ganglions neurons, but the IgG low-affinity activating receptor FcγRIII were only expressed on the satellite glial cells, not neurons of mouse trigeminal ganglions. The only IgE high-affinity activating receptor FcεRI was just expressed on trigeminal ganglions neurons, but the IgE low-affinity receptor FcεRII was expressed on neurons and satellite glial cells of trigeminal ganglions. Compare with na?ve mouse, the level of serum total IgE and the FcεRI and FcγRII protein of trigeminal ganglions were increased in allergic mouse. But the protein levels of trigeminal ganglions FcεRII and FcγRI were decreased in allergic mouse. The expression of FcγRIII was no significantly difference between na?ve mouse and allergic mouse trigeminal ganglions. Conclusion Fcγ and ε receptor on mouse trigeminal ganglions may involve in the allergic desease.

Key words: Fcγ receptor, Fcε receptor, IgG, IgE, Allergy

中图分类号: