关键词: IL-25, 哮喘, Nuocytes, IL-5, IL-13

Abstract: Objective To study the effect of Interleukin-25 on expressing Th2 inflammatory factor in asthma by nuocyte cells. Methods Nuocyte were collected from mouse spleen of OVA-induced asthma model by density gradient centrifugation. Hematoxylin-eosin staining and pulmonary function tests were determined OVA-induced asthma model to be established successfully from the pathology and Physiology; Samples were divided into 3 groups according to different intervention conditions: Interleukin-25 group; control group; anti-Interleukin-25 group. The expression of Interleukin-5 and Interleukin-13 proteins in nuocyte cells supernatant was detected by ELISA. The expression of Interleukin-5 and Interleukin-13 proteins in nuocyte cells was detected by Western blot. The expression of Interleukin-5 and Interleukin-13 mRNA was detected by RT-PCR. The number of nuocyte cells was detected by flow cytometry. Results The study showed that expression of Interleukin-5 and Interleukin-13 protein and related gene in Interleukin-25 group was higer than that in the control group or anti-Interleukin-25 group (P＜0.05). Flow cytometry showed that the number of nuocyte cells in Interleukin-25 group was higer than that in control group or anti-Interleukin-25(P＜0.05). Conclusions Interleukin-25 can promote the expressing Interleukin-5 and Interleukin-13 in asthma by nuocyte cells and promote the occurrence and development of asthma.

Key words: IL-25, Asthma, Nuocytes, IL-5, IL-13