基础医学与临床 ›› 2014, Vol. 34 ›› Issue (7): 974-978.

• 研究论文 • 上一篇    下一篇

siRNA沉默CD55基因对胰腺癌BxPC-3细胞增殖的影响

何政1,吴慧1,万荣华1,郑军2   

  1. 1. 湖北省宜昌中心人民医院
    2. 三峡大学第一临床医学院
  • 收稿日期:2013-11-11 修回日期:2014-03-25 出版日期:2014-07-05 发布日期:2014-06-24
  • 通讯作者: 郑军 E-mail:zhengjun@163.com

Effect of siRNA-mediated silencing of the decay accelerating factor(CD55) on biological behaviour of human pancreatic cancer cell line BxPC-3.

  • Received:2013-11-11 Revised:2014-03-25 Online:2014-07-05 Published:2014-06-24

摘要: 目的 探讨小分子干扰RNA(siRNA)沉默衰亡加速因子(CD55)基因对胰腺癌细胞增殖的影响。方法:针对CD55基因设计3对siRNA(阴性对照siRNA由广州锐博公司直接提供),转染胰腺癌BxPC-3细胞,用实时定量PCR和Western blot检测CD55mRNA和蛋白的表达,并用流式细胞术检测细胞生物学行为的改变。 结果 转染BxPC-3细胞48h后,3条siRNA中仅siRNA3能显著抑制CD55mRNA和蛋白的表达,细胞凋亡比率显著增加(P<0.05);细胞增殖率显著减少(P<0.05);细胞G1/G0期升高,G2期变化不明显,S期下降(P<0.05)。结论 使用siRNA能够有效地沉默CD55基因的表达,并显著影响胰腺癌细胞的生物学行为。

关键词: 胰腺癌, RNA干扰, CD55, 细胞凋亡, 细胞增殖, 细胞周期

Abstract: To study the specific effect of silencing of CD55 gene by small interfering RNA (siRNA) on the growth of human pancreatic cancer cell line BxPC-3. Methods Three CD55-specific siRNAs and one negative siRNA were designed and was transfected into human pancreatic cancer cell line BxPC-3 by Lipofectamine 2000.Quantitative real-time PCR(qRT-PCR) and Western blot were used to test the expression levels of CD55 mRNA and protein respectively. And flow cytometry were used to detect apoptosis, proliferation and cell cycle of target cells. Results: One of three CD55-specific siRNAs, siRNA3 could silence the expression of the CD55 gene. The expression levels of CD55 mRNA and protein decreased remarkably.The cells transfected with siRNA3 were found to increase apoptosis and reduce proliferation significantly;the target cells stagnated in phase G1 after transfection(P<0.05). Conclusion: SiRNA-mediated silencing of the decay accelerating factor(CD55) can effectively affect the biological behavior of human pancreatic cancer cell line BxPC-3.

Key words: Pancreatic carcinoma, RNA interference, CD55, Cell apoptosis, Cell proliferation, Cell cycle

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