TBB通过WNT/β-CATENIN通路抑制甲状腺滤泡状癌细胞FTC133的增殖与迁移

基础医学与临床 ›› 2014, Vol. 34 ›› Issue (10): 1381-1385.

TBB通过WNT/β-CATENIN通路抑制甲状腺滤泡状癌细胞FTC133的增殖与迁移

王翠瑶¹,刘超²,任丽莉¹,张晓玉¹,孙静¹,肖建英¹

1. 辽宁医学院基础医学院
2. 辽宁医学院

收稿日期:2014-02-21 修回日期:2014-05-24 出版日期:2014-10-05 发布日期:2014-09-25
通讯作者: 肖建英 E-mail:lyxiaojy@163.com
基金资助:
辽宁省科学技术计划项目

TBB inhibits the proliferation and migration of thyroid papillary carcinoma cell line FTC133 via WNT/β-CATENIN signaling pathway

Received:2014-02-21 Revised:2014-05-24 Online:2014-10-05 Published:2014-09-25

摘要/Abstract

摘要： 目的观察四溴苯三唑(TBB)对甲状腺滤泡状癌细胞株FTC133的CK2ɑ、β-CATENIN和SURVIVIN mRNA和蛋白表达的影响，探讨TBB抗甲状腺癌的作用机制。方法体外培养FTC133细胞，分对照组（DMSO组）和TBB组（终浓度为12.5、25和50μmol/L），MTT法测定细胞增殖抑制率；划痕实验测定细胞迁移；RT-PCR和Western blot法分别检测FTC133细胞CK2ɑ、β-CATENIN和SURVIVINmRNA和蛋白表达。结果与对照组比较，TBB呈剂量依赖性抑制FTC133细胞的增殖和迁移。各TBB处理组CK2ɑmRNA表达水平明显降低（P<0.01）；TBB组随着浓度的逐渐加大，CK2ɑ、β-CATENIN和SURVIVIN蛋白表达下降（P<0.01）。结论TBB在一定浓度范围内抑制甲状腺滤泡状癌细胞株FTC133的增殖和迁移，其机制可能与TBB下调CK2ɑ表达进而抑制WNT/β-CATENIN信号通路有关。

关键词: 四溴苯三唑, 甲状腺滤泡状癌, CK2ɑ, β-CATENIN, SURVIVIN

Abstract: Objective　To observe the effects of 4,5,6,7-tetrabromobenzotriazole(TBB) on the expressions ofCK2ɑ，β-CATENIN and SURVIVINin thyroid papillary carcinoma cell line FTC133and to explore the possible underlyingmechanism. Methods FTC133 cellswere cultured in vitro and divided into control group(DMSO), TBBgroups(12.5, 25, 50μmol/L)randomly. The proliferationrateand migration of FTC133 cells was detected byMTTand wound healing method. TheCK2ɑ, β-CATENIN and SURVIVIN mRNA and protein expression levelswere determined by RT–PCR and Western blot. Results Compared with control group, the proliferationrate and migration of FTC133 cells wereinhibited by TBBin a dose–dependentmanner(P<0.01).The expression levels of mRNA and protein ofCK2ɑ were significantly decreased in the presence of different TBB in FTC133 cells（P<0.01）, the protein expression level of β-CATENIN and SURVIVIN were decreased, too（P<0.01）.Conclusion TBB inhibits the proliferation and migration of thyroid papillary carcinoma cell line FTC133 via WNT/β-CATENIN signaling pathway.

Key words: TBB, thyroid papillary carcinoma, CK2ɑ, β-CATENIN, SURVIVIN

王翠瑶刘超任丽莉张晓玉孙静肖建英. TBB通过WNT/β-CATENIN通路抑制甲状腺滤泡状癌细胞FTC133的增殖与迁移[J]. 基础医学与临床, 2014, 34(10): 1381-1385.

[1]	陈思文进李方方雷占翔. Exendin-4促进小鼠脂肪干细胞向成骨细胞分化[J]. 基础医学与临床, 2019, 39(8): 1152-1156.
[2]	王思敏周晴王倩梁元晶翁静马伟. 小鼠卵母细胞减数分裂过程中PAK1与survivin的亚细胞定位和蛋白水平相关[J]. 基础医学与临床, 2019, 39(5): 673-676.
[3]	孙莹梁晓春刘伟刘頔. 筋脉通含药血清对高糖培养大鼠雪旺细胞β-catenin、GSK-3β及髓鞘零蛋白表达的影响[J]. 基础医学与临床, 2017, 37(4): 484-487.
[4]	王彬彬武承凤张方信. 自噬在肠黏膜屏障功能作用机制的研究进展[J]. 基础医学与临床, 2017, 37(3): 405-409.
[5]	李红丽芦永良申利红冯涛黄佳祎. Wnt/β-catenin信号途径抑制Raw264.7分化[J]. 基础医学与临床, 2013, 33(9): 1155-1159.
[6]	仇黎丽徐青徐昌芬. 左炔诺孕酮下调Survivin基因促进人子宫肌瘤细胞凋亡[J]. 基础医学与临床, 2012, 32(8): 926-929.
[7]	陈筠孙莉. Wnt /β-catenin信号传导通路与早期胚胎心脏发育的关系[J]. 基础医学与临床, 2010, 30(1): 103-106.
[8]	丁浩何柳兴冯涛. 乙肝病毒X蛋白对人肝细胞内β-catenin定位及转录活性的影响[J]. 基础医学与临床, 2009, 29(5): 475-478.
[9]	王学虎傅仲学谌伟吴星烨. VEGF上调不同类型细胞Survivin的表达[J]. 基础医学与临床, 2009, 29(12): 1239-1243.