关键词: 垂体肿瘤转化基因1, 上皮-间质转化, 肺肿瘤, 侵袭

Abstract: Objective To investigate the role of PTTG1 in the migration and invasion of Non-small cell lung cancer. Methods To detect the expression of PTTG1, E-cadherin and Vimentin in 80 cases of NSCLC using immunohistochemistry. Cells were divided by four groups : ①A549/con, cells were transfected by empty vector②A549/PTTG1, cells were transfected by purpose gene ③Scr/H1299, cells were transfected by empty vector ④SiPTTG1/H1299, cells were knocked down purpose gene. Western blot was used to analyze the expression of PTTG1, E-cadherin and Vimentin. Results The protein expression of PTTG1 in NSCLC was negatively correlated with E-cadherin and positively correlated with Vimentin (P<0.05). H1299 cells expressed higher PTTG1, and A549 cells expressed lower PTTG1. The expressions of PTTG1 and Vimentin were lower in SiPTTG1/H1299 cells than those in Scr/H1299 and H1299 cells after trancfected 24 hours, but the E-cadherin expression was higher. At the same time, the protein expression of Vimentin was up-regulated and the expression of E-cadherin was down-regulated in A549/PTTG1 cells. Conclusions PTTG1 can promote migration and invasion of NSCLC through EMT.

Key words: PTTG1, EMT, Lung tumor, migration